摘要
目的探讨端粒酶逆转录酶(TERT)、端粒酶相关蛋白1(TP1)和端粒重复结合因子2(TRF2)表达与心肌细胞分化和发育的关系。方法应用S-P免疫组化方法分别对不同月份胎儿心肌细胞TERT、TP1和TRF2蛋白表达进行检测。结果胎儿心肌细胞核中TERT的表达随胎龄增加而减少;胎儿心肌细胞胞浆TP1和TRF2的表达随胎龄增加而增加。结论TERT、TP1和TRF2表达方式可能促使部分心肌细胞永久退出细胞周期而进入终末分化和肥大阶段。
Objective To investigate protein expression of telomerase reversetranscriptase（TERT）, telomerase - associated protein l （TP1） and telomeric repeat binding factor 2（TRF2） in human fetal cadiacmyocytes. Methods Using immunohistochemical method,sections of human fetal hearts were stained with antibodies against TERT, TP1 and TRF2. Results Expression of TERT was gradually decreased with development in human fetal cadiacmyocytes nuclear. Expression of TP1 and TRF2 was gradually increased with development in human fetal cadiacmyocytes cytoplasm. Conclusion Down - regulation of TERT and up - regulation of TP1 and TRF2 may promote the permanent withdrawal of cardiomyocytes from the cell cycle and enter terminal differentiation and myocardiurn hypertrophy.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2006年第1期36-37,共2页
Journal of Applied Clinical Pediatrics
基金
河南省教育厅自然科学基金项目资助(200510472003)
关键词
端粒酶逆转录酶
端粒酶相关蛋白1
端粒重复结合因子2
心肌
telomerase rever
transcriptase
telomerase - associated protein
telomeric repeat binding factor 2
myocardium
