OX34预防大鼠小肠移植急性排斥反应

Role of OX34 in treatment of acute rejection after small bowel transplantation in rats

目的:探讨可溶性抗CD2 mAb(OX34)在大鼠小肠移植(SBT)急性排斥反应中的治疗作用.方法:将两种近交系大鼠(SD,W istar)72只随机分为A,B,C,D 4组,A组为对照组(n=18)行虚拟手术,给予普通饲料喂养;B组(n=18)行同系小肠移植(iso-strain SBT),术后常规补液,给予抗生素;C组(n=18)行不同品系小肠移植(aniso-strain SBT),术后处理同B组;D组(n=18)行不同品系小肠移植,术后除常规补液、给予抗生素外,同时给予OX34.各组于术后3,5,7 d取材.行病理学及流式细胞术(FCM)检测.结果:手术后C组移植受体存活时间及外周血CD2阳性T淋巴细胞表达率较其他3组差异有统计学意义(P<0.05).移植肠病理学检查均有从轻度至重度急性排斥反应的发生.D组部分受体于术后5,7d出现轻度急性排斥反应.A,B组未见明显排斥反应发生.结论:OX34可有效抑制急性排斥反应的发生.

AIM： To investigate the therapeutic effect of OX34 against acute rejection after small bowel transplantation （SBT） in rats. METHODS ： A total of 72 rats of 2 inbred lines （ SD and Wistar） were randomly divided into 4 groups（ A, B, C and D）. Group A （n = 18） accepted fictitious operation and was fed with common forage; Group B （n = 18） underwent iso-strain SBT and the treatment of post-operation such as routine fluid replacement and antibiotic administration; Group C （n = 18） had aniso-strain SBT and the same post-operative treatment as group B; and Group D （ n = 18 ） got the same operation as group C and OX34 was added into the same post-operative treatment as group B. Samples of grafts and peripheral blood were taken from 3, 5 and 7 d after SBT and were detected by pathology and flow cytometer. RESULTS： The survival time and the positive expression rate of CD2 in peripheral blood of group C were significantly different compared with those of the other 3 groups （ P 〈 0. 05 ）. Acute rejection developed from mild to severe when the samples were taken form 3, 5, 7 d after SBT. Part of the grafts from group D suffered from a mild degree of acute rejection 5 and 7 d after SBT, while none of the group A and B showed pathological evidence of acute rejection. CONCLUSION： OX34 significantly suppresses the acute rejection after SBT in vivo.