PDGF-AB对成纤维细胞表达α-SM actin的影响

Effect of PDGF-AB on α-SM actin expression of fibroblasts

目的:研究PDGF-AB对成纤维细胞表达-αSM ac-tin的影响,探讨PDGF-AB促进伤口愈合的作用机制.方法:取体外培养的人正常皮肤成纤维细胞,用免疫组化(SABC)观察PDGF-AB对成纤维细胞表达α-SM actin的影响.结果:PDGF-AB能诱导成纤维细胞表达-αSM actin,且呈剂量依赖性关系.结论:PDGF-AB可能通过刺激成纤维细胞表达α-SM actin,促进成纤维细胞向肌成纤维细胞转化,增加伤口收缩,促进伤口愈合.

AIM： To study the effect of PDGF-AB on α-SM actin expression of fibroblasts and to investigate the mechanism by which PDGF-AB promotes wound healings. METHODS： In vitro cultured human normal skin fibroblasts were applied to measure the effect of PDGF-AB on α-SM actin expression by immunohisto- chemistry （SABC）. RESULTS： PDGF-AB could enhance α-SM actin expression in a dose-dependent manner. CONCLUSION： PDGF-AB may promote wound healing through stimulating wound contraction by enhancing α-SM actin expression of fibroblasts and stimulating fibroblasts to transform into myofibroblasts.