结直肠癌中Cks1,P27^(Kip1)和Skp2蛋白的表达

Cks1,P27^(Kip1) and Skp2 protein expression in colorectal cancer

目的:探讨Cks1在结直肠癌发生及其在Skp2调节P27K ip1降解过程中的作用.方法:应用流式细胞术测定正常结直肠黏膜、结直肠腺瘤和结直肠癌组织中Cks1,P27K ip1和Skp2蛋白的表达.结果:由正常结直肠黏膜、结直肠腺瘤到结直肠癌Cks1,Skp2表达呈上升趋势(P<0.05),P27K ip1表达呈下降趋势(P<0.05).结直肠癌中Cks1,Skp2表达与P27K ip1表达呈负相关(r=-0.752,P<0.05;r=-0.746,P<0.05);Cks1与Skp2呈正相关(r=0.845,P<0.05).结直肠癌中Cks1的表达与肿瘤分化程度相关(P<0.05),而与淋巴结转移及Dukes分期不相关(P>0.05).结论:Cks1可能参与了结直肠癌的发生;结直肠癌中Cks1可能参与了Skp2调节P27Kip1泛素化降解过程.

AIM： To investigate the expression of Cksl protein and its relationship with the expressions of Skp2, P27^kip1 and colorectal tumorigenesis. METHODS： Flow cytometry was used to detect the expressions of Cksl, Skp2 and P27^Kip1 in 20 normal colorectal mucosas, 20 adenomas as well as 54 colorectal cancers. RESULTS： The expressions of Cksl and Skp2 gradually increased in the order of normal mucosa, adenoma to colorectal cancer （ P 〈 0.05 ） , while the expression of P27^Kip1 gradually decreased in the same order （ P 〈 0.05 ）. A strong correlation was found between the expressions of Cksl and Skp2 （r = 0. 845, P 〈 0.05 ） and a significant inverse relation was also observed between the expressions of Cksl or Skp2 and of P27^kip1 （ r = -0. 752, P 〈 0.05 ; r = - 0. 746, P 〈 0.05 respectively）. The expression of Cksl was significantly associated with the loss of tumor differentiation （P 〈0.05 ） , but not with lymph node metastasis and Dukes＇ staging （P 〉 0. 05 ）. CONCLUSION： Cksl may play an important role in human colorectal tumorigenesis. Cksl may be an essential factor in proteasome-dependent degradation of P27^kip1 regulated by Skp2.