超声造影剂介导PTEN基因影响卵巢癌侵袭的实验研究

Ultrasound-mediated PTEN gene inhibit invasion and metastasis of human ovarian carcinoma SKOV3 cell lines

目的研究超声介导造影剂增效PTEN基因在卵巢癌细胞中的转染,探讨该基因抑制肿瘤细胞侵袭的机制。方法分别于接种人卵巢黏液性囊腺癌细胞(SKOV3)的6孔板中每孔加入200μl造影剂和5μl脂质体,以诊断超声剂量辐照60s,细胞转化48h后,采用重组基底膜侵袭模型,观察转染前后SKOV3细胞侵袭力的变化。结果转染PTEN基因可以明显抑制肿瘤细胞的侵袭力。结论PTEN基因可以通过抑制肿瘤细胞侵袭力而抑制卵巢肿瘤的生长;超声介导造影剂增效基因转染,可望成为临床基因治疗的新手段。

Objective To determine the transfection of PTEN gene in human ovarian carcinoma SKOV3 cells by ultrasound mediating and contrast agent＇s enhancing, and to explore the mechanism of PTEN gene inhibiting the tumor cells＇ invasion. Methods After addition of PTEN gene and 5μl lipofectamine,human ovarian carcinoma SKOV3 cells on 6-well plates were explored to ultrasound for 60 s in the presence （200μl） of ultrasound agent. The invasiveness of transfected cells was measured quantitatively by Matrigel invasion assays （Transwell chamber） after 48 h. Results The invasiveness of SKOV3 cells transfected by ultrasound mediated PTEN gene was significantly declined compared with that of the controls. Conclusions The transfection of PTEN gene into SKOV3 cell can inhibit their invasion. The gene transfection can be enhanced by ultrasound and contrast agent,which may he expected to he a new means of clinical gene therapy.