不同程度牙周炎自然发展进程与IL-1RN基因多态性的关系

The progress of chronic periodontitis and its association with IL-1RN genetic polymorphisms

目的了解慢性牙周炎病程的自然发展及其与IL-1RN(INTRON2)VNTR基因多态性的关系。方法对110例慢性牙周炎患者,其中轻度18例,中度34例,重度58例进行为期6个月的自然病程观察,分别在基线、6个月对全口余留牙牙周袋深度PD、临床附着丧失量AL进行检查,每个牙检查6个位点并检测其IL-1RN(INRON2)VNTR的基因多态性。结果110人平均牙周袋深度增加PD=0.23mm,平均附着丧失量增加AL=0.50mm。其中36例患者44个牙位出现了△AL≥2mm。6个月后PDAL的改变在IL-1RN(INTRON2)VNTR基因型1/1携带者与基因型1/2携带者间无明显差异(P>0.05)。6个月后IL-1RN(INTRON2)VNTR基因型及等位基因频率在△AL≥2mm组和病情无明显改变组无统计学差异(P>0.05),重度牙周炎组较轻中度牙周炎组更易出现快速进展(P>0.05)。结论不同个体、不同程度其牙周病变的发展是不一样的,还不能认为IL-1RN(INTRON2)VNTR基因多态性会影响汉族人群慢性牙周炎的发展。

Objective To study the natural progress of chronic periodontitis and its association with 1L-1 RN genetic polymorphisms. Methods 110 subjects with chronic periodontitis （mild 18, moderate 34, severe 58 ） were examined at baseline and 6 month follow-up visit on probing depth, attachment level on 6 sites of each tooth. IL-1 genetic polymorphisms were genotyped with PCR-RFLP. DNA samples were obtained with buccal swabbing technique. Results Among the 110 subjects, 36 patients had 44 sites with ≥2mm attachment loss over the 6 month period. The mean PD, AL increased were 0.2mm and 0.5mm, respectively. There was no significant difference in the PD and the AL changes at the 6 month visit between the IL-1 RN 1/1 carriers and the IL-1 RN 1/2 carrieres. There was no significant difference in the frequency distribution of IL-1RN （intron2）, VNTR genotypes, and alleles between the rapidly progressive group （with AL≥2mm at least one site） and the non-rapidly progressive group. Conclusion The progress of chronic periodontitis varies individually. IL-1RN （INTRON2） VNTR genetic polymorphisms may not significantly influence the progress of chronic periodontitis.