应用人自体血清培养人口腔黏膜上皮的实验研究

An experimental study on the culture of human oral mucosa epithelium using autologous serum

目的研究人自体血清培养人口腔黏膜移植生长的生物学特性,为组织工程化尿道提供新材料。方法将人自体血清培养黏膜移植于裸鼠体内,分别于移植后2、3、4、6周观察培养黏膜生长与转归,应用anti-HLA免疫荧光鉴定成活黏膜组织属性,应用抗人Ⅳ型胶原及抗人层黏蛋白为基底膜形成指标。结果裸鼠体内移植培养黏膜成活生长分化良好,anti-HLA免疫荧光证实为移植的培养人黏膜组织;免疫组化发现移植后3周开始形成基底膜,4周形成完整的基底膜。结论自体血清培养的人口腔黏膜可形成功能完整的上皮组织。

Objective To investigate the biological properties of cultured human oral mucosa epithelium using autologous serum in order to provide a new material for tissue engineering urethra. Methods The cultured oral mucosa epithelium was respectively transplanted beneath the skin, above the deep fascia and in the wound of the athymic mice. The specimens were taken at 2, 3,4, and 6 weeks posttransplantation, and processed for ① immunofluorescence anti-HLA staining to determine graft acceptance, and ② anti-human IVcollagen and antihuman laminin immunohistochemical staining procedures to indicate the basement membrane formation. Results All the grafts survived and grew very well. The grafts were positive to anti-HLA. Collagen type IV and laminin were detected at the dermo-epidermal junction in all groups from day 14, and increasing in density up to day 21. Conclusions The cultured human oral mucosa epithelium by autologous serum could develop an excellent functional epithelium tissue, which would be used to reconstruct urethra and repair wound.