HaSNPV的9个基因的转录谱分析

The Transcription Profiles of Nine HaSNPV Genes

使用反转录和实时荧光定量PCR技术,我们对HaSNPV的几个预期的早期基因、早晚期基因、晚期基因、极晚期基因的转录时相进行分析,结果表明:这些基因的起始转录时间与其自身的启动子类型基本是一致的。但是预期的早期基因pkip晚期才开始转录;预期的早晚期基因ha107早期不转录,仅在晚期转录。这些基因的转录水平一般都在病毒感染细胞72h后达到最高,并且极晚期基因polyhedrin的转录水平明显高于其它基因。Iap2的转录水平仅次于polyhedrin,表明它可能是一个功能基因。与AcMNPV的p10不同,在HaSNPV/HzAM1系统中p10的转录水平并不高。

The transcription profiles of nine selected HaSNPV genes, e.g. early phase genes, early and late phase genes, late phase genes and very late phase genes were analyzed using reverse transcription and real-time quantitative PCR. The results indicated that for most of these genes the transcriptional start point was coincident with the prediction of their promoter. But the predicted early phase gene pkip started to be transcribed at late phase, and the predicted early and late phase gene hal07 was only transcribed at late phase. At 72hpi, the transcription level of these genes was generally the highest. The transcription level of polyhedrin was obviously higher than the other genes. The iap2 was the second highly transcribed gene, indicating that it might be functional. The HaSNPV plO, unlike its homologue in AcMNPV, was not highly transcribed.