摘要
The spike gene(S)of Infectious bronchitis virus(IBV)CK/CH/LDL97Ⅰ/97 isolate was amplified,cloned,sequenced and analyzed.Results showed that the S gene was 3501 bp encoding a polypeptide of 1167 amino acids.The precursor of the S protein was cleaved into S1 and S2 fragments,which comprised 541 and 626 amino acid residues,respectively.The cleavage site sequence was RRTGR.The homologies of nucleotide and amino acid sequences of S1 gene between CK/CH/LDL97 Ⅰ/97 and 27 reference IBV strains ranged from 60.6% to 99.6%,and 50.5% to 99.1%,respectively.The CK/CH/LDL97Ⅰ/97 S1 was most similar to that of three proventriculus IBV isolates,Q1(99.1%),J2(98.9%),T3(98.9%),which were isolated in China.Phylogenetic analysis of S1 proteins indicated that CK/CH/LDL97Ⅰ/97,Q1,J2 and T3 formed a separated cluster,which might belong to a novel genotype of IBV.
The spike gene (S) of Infectious bronchitis virus (IBV) CK/CH/LDL97 Ⅰ/97 isolate was amplified, cloned, sequenced and analyzed. Results showed that the S gene was 3501 bp encoding a polypeptide of 1167 amino acids. The precursor of the S protein was cleaved into S1 and S2 fragments, which comprised 541 and 626 amino acid residues, respectively. The cleavage site sequence was RRTGR. The homologies of nucleotide and amino acid sequences of S1 gene between CK/CH/LDL97 Ⅰ/97 and 27 reference IBV strains ranged from 60.6% to 99.6%, and 50.5% to 99.1%, respectively. The CK/CH/LDL97 Ⅰ/97 S1 was most similar to that of three proventriculus IBV isolates, Q1(99.1%), J2(98.9%), T3 (98.9%), which were isolated in China. Phylogenetic analysis of S1 proteins indicated that CK/CH/LDL97 I/97, Q1, J2 and T3 formed a separated cluster, which might belong to a novel genotype of IBV.
出处
《中国病毒学》
CAS
CSCD
2006年第1期68-70,共3页
Virologica Sinica
基金
黑龙江省政府博士后科研启动金资助项目(LRB-KY01045)
关键词
传染性支气管炎病毒
S糖蛋白
基因特征
Infectious bronchitis virus
Spike glycoprotein
Molecular characterization