摘要
目的研究脑胶质细胞瘤病人外周血中白细胞介素2(IL-2)、可溶性白细胞介素2受体(sIL-2R)的表达以及红细胞免疫和T淋巴细胞亚群的变化规律,探讨它们之间的相互关系。方法对55例脑胶质瘤病人及55例健康献血员,采用酶联免疫吸附(ELISA)法测定血清IL-2s、IL-2R含量,免疫黏附法测定红细胞免疫活性及其调节功能,链亲和素-过氧化物酶(SP)一步法测定CD3、CD4、CD8细胞数。结果脑胶质细胞瘤组IL-2含量较对照组显著性降低(P<0.01),sIL-2R则显著性升高(P<0.01);红细胞C3b受体(RBC-C3bR)、红细胞免疫调节促进因子(RFER)亦显著性降低(P<0.01),而红细胞免疫复合物(RBC-ICR)、红细胞免疫调节抑制因子(RFIR)则显著性升高(P<0.01);CD3、CD4细胞数显著性降低(P<0.001),而CD8无显著性差异(P>0.05)。结论脑胶质细胞瘤病人存在免疫功能低下;检测血清IL-2和sIL-2R含量、红细胞免疫功能及T细胞亚群活性,对脑胶质细胞瘤病人的免疫机制研究具有重要意义。
Objective To study the alteration in expressions of interleukin-2 （IL-2） and soluble interleukin 2 receptor （sIL-2R）, erythrocyte immune and T cell subgroup in the peripheral blood in the patients with cerebral glioma, and explore the relationships between them. Methods In 55 patients with cerebral gliomas and 55 healthy blood donors, ELISA was used to determine the contents of IL-2 and sIL-2R, immunoadsorption employed to examine the erythrocyte immune activity and its regulatory function, and streptavidin-peroxidase immunohistochemical method used to determine cell numbers of CD3 （cluster of differentiation 3）, CD4, and CD8. Results The content oflL-2 in cerebral glioma group was significantly lower than that in the control group （P〈0.01） and that of slL-2R increased significantly （P〈0.01）. RBC-C3b receptor and RBC immune adherence-enhancing factor dropped markedly （P〈0.01）, while RBC immune complex rosette and RBC immune regulation-inhibiting factor were greatly increased （P〈0,01）. The cell number of CD3, CD4 decreased （P〈0.001）, and there was no evident change of CD8 （P〉0.05）. Conclusions Suppression of immune function was observed in the patients with cerebral glioma. The determination of IL-2 and sIL-2R levels, immune function of WBC and activity of T subgroup has important significance in study of immune system in oatients with cerebral glioma.
出处
《中国微侵袭神经外科杂志》
CAS
2006年第1期18-20,共3页
Chinese Journal of Minimally Invasive Neurosurgery
基金
陕西省科技厅科技攻关项目(2002K10-G6)
