氧化低密度脂蛋白对THP-1巨噬细胞CD36表达的影响

Effect of oxidized low density lipoprotein on CD36 expression in macrophage

目的:观察氧化低密度脂蛋白(oxLDL)对THP-1巨噬细胞CD36表达和细胞胆固醇流入的影响,探讨CD36在巨噬细胞胆固醇流入中的作用。方法:用50 mg/L oxLDL分别孵育THP-1巨噬细胞不同时间(0、12、24、36和48 h),采用逆转录-聚合酶链式反应(RT-PCR)和W estern印迹分别检测CD36 mRNA和蛋白质的表达;用油红O染色观测细胞内脂质蓄积情况;用液体闪烁计数法检测[3H]胆固醇流入。结果:50mg/L oxLDL孵育THP-1巨噬细胞不同时间(0、12、24、36和48 h),THP-1巨噬细胞CD36 mRNA及蛋白质表达上调,油红O染色可见细胞内脂滴逐渐增加,液体闪烁计数发现THP-1巨噬细胞胆固醇流入增加,分别为23.5%、27.8%、39.7%、44.1%和49.7%。结论:oxLDL可使THP-1巨噬细胞CD36表达上调,并使细胞胆固醇流入增加。

Objective： To investigate the effect of oxLDL on CD36 expression and cholesterol influx in THP-1 macrophage. Methods： THP1 macrophage were treated with 50mg/L oxLDL for 0, 12, 24, 36, 48 h respectively. Oil red O staining was used to observe the intracellular lipid droplets. [^3H ] labeled Cholesterol influx was determined by FJ-2107P type liquid scintillator. CD36 mRNA and protein level were determined by reverse transcription-polymerase chain reaction（RT-PCR） and Western blotting respectively. Results：We found that oxLDL elevated CD36 in both protein and mRNA levels and increased cholesterol influx in a time-dependent manner. The levels of cholesterol influx were 23.5% , 27.8% ,39.7% ,44.1% and 49.7% respectively. Conclusion ： oxLDL may upregulate CD36 expression and increase cholesterol influx.