摘要
目的研究黄蜀葵花总黄酮(Total F lavone of Abelmos-chusM an ihot(L)M ed ic,TFA)药理性预适应对家兔心肌缺血再灌注损伤的保护作用及机制。方法采用持续静脉推注TFA模拟预适应的实验方法,观察家兔冠状动脉左旋支经结扎、再灌后,其心肌组织病理损伤情况、血浆中MDA含量和SOD、GSH-PX活力的变化以及心肌组织中细胞间粘附分子ICAM-1的表达情况。结果TFA不同程度减轻缺血再灌注损伤家兔心肌病理损伤的严重程度;明显降低血浆中MDA的含量,同时增强SOD及GSH-PX的活力;下调缺血心肌组织中ICAM-1 mRNA的表达。结论TFA药理性预适应可通过抑制心肌脂质过氧化、抑制心肌炎症反应,对缺血再灌注损伤的心肌具有保护作用。
Aim To observe the protective effect and possible mechanisms of Total Flavone of Abelmoschus Manihot L medic （TFA） on myocardial reperfusion injury in rabbits, when it was given as a preconditioning inducer. Method Different doses of TFA were perfused intravenously lasting for 5 min, followed by a 10 min break before 30 min ischemia and 60 min reperfusion in rabbits. Using this preconditioning method, we observed the histo-pathological changes of myocardium, the content of MDA, the activities of SOD and GSH-PX in plasma, and also, the expression of ICAM- 1 in myocardium. Results TFA（ 16,8 and 4 mg ·kg^-1 ） significantly ameliorated myocardium ischemical reperfusion injury. The activities of SOD and GSH-PX in plasma were enhanced, while the MDA level was decreased. Meanwhile, the expression of ICAM-1 mRNA in myocardium was down-regulated. Conclusion Pharmacological preconditioning of TFA could protects myocardial ischemic reperfusion injury in rabbits by inhibiting lipid peroxidation and myocardium inflammatory reaction.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2006年第1期106-109,共4页
Chinese Pharmacological Bulletin
关键词
TFA
药理性预适应
心肌缺血再灌注
脂质过氧化
ICAM-1
TFA
pharmacological preconditioning（PP）
ischemical reperfusion injury （IRI）
lipid peroxidation
ICAM-1
