摘要
目的:观察脑还丹对快速老化小鼠学习、记忆的影响及其抗氧化、抗凋亡作用。方法:将40只SAM-P/8快速老化小鼠随机分为4组:模型组、银可络组、脑还丹低剂量组和脑还丹高剂量组,每组10只。模型组予以生理盐水灌胃,其余各组予以相应药物治疗。治疗105d后,采用跳台实验及Y型迷宫实验进行小鼠行为学测试,并检测小鼠血清超氧化物歧化酶(superoxidedismutase,SOD)、丙二醛(malondialdehyde,MDA)、谷胱甘肽过氧化物酶(glutathioneperoxidase,GSH-Px)的含量。采用逆转录聚合酶链反应(reversetranscription-polymerasechainreaction,RT-PCR)检测小鼠大脑皮质及海马凋亡抑制基因bcl-xlmRNA的表达。结果:银可络组和脑还丹低、高剂量组小鼠的学习、记忆成绩均明显优于模型组(P<0.05或P<0.01),其中以脑还丹高剂量组的疗效最为显著(P<0.01)。银可络组和脑还丹低、高剂量组小鼠血清SOD、GSH-Px的含量及大脑皮质和海马bcl-xlmRNA的表达,均明显高于模型组(P<0.05或P<0.01);而血清MDA的含量则明显低于模型组(P<0.01)。结论:脑还丹能改善快速老化小鼠的学习、记忆能力,具有抗氧化及促进bcl-xlmRNA表达的作用。
Objective: To investigate the effects of Naohuandan Recipe on learning and memory abilities of SAM-P/8 mice and its role in anti-oxidation and anti-apoptosis. Methods: Forty SAM-P/8 mice were randomly divided into four groups, which were untreated (normal saline-treated) group, Yinkeluo Tablets (extracts of gingko leaf)-treated group, low-dose Naohuandan Recipe-treated group and high-dose Naohuandan Recipetreated group. Mice in these groups were given corresponding drugs orally for 105 days. Then the performances of learning and memory of mice were tested by a step-down passive avoidance task and a Y-maze test. The serum levels of superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) were detected. The expression level of bcl-xl mRNA in cerebral cortex and hippocampus of mice was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results: The performances of learning and memory in the Yinkeluo Tablets-treated group, low- and high-dose Naohuandan Recipe-treated groups were significantly improved as compared with those in the untreated group (P〈0.05 or P〈0.01), and such performance was the best in the high-dose Naohuandan Recipe-treated group among these four groups (P〈0.01). The serum levels of SOD and GSH-Px and the expression of bcl-xl mRNA in cerebral cortex and hippocampus of mice in the Yinkeluo Tablets-treated group, low- and high-dose Naohuandan Recipe-treated groups were also significantly higher than those in the untreated group ( P 〈 0. 05 or P〈0.01), while the serum level of MDA in the untreated group was higher than that in the other three groups (P〈0.01). Conclusion: Naohuandan Recipe can improve learning and memory abilities of SAM-P/8 mice, and this effect may be related to its anti-oxidation efficacy and enhancement of expression level of bcl-xl mRNA.
出处
《中西医结合学报》
CAS
2006年第1期48-51,共4页
Journal of Chinese Integrative Medicine
基金
广东省中医药管理局基金资助项目(No.A002003002)