脑还丹对快速老化小鼠学习记忆能力的影响及其抗氧化和抗凋亡作用

Effects of Naohuandan Recipe on learning and memory abilities of SAM-P/8 mice and its role in anti-oxidationand anti-apoptosis

目的:观察脑还丹对快速老化小鼠学习、记忆的影响及其抗氧化、抗凋亡作用。方法:将40只SAM-P/8快速老化小鼠随机分为4组:模型组、银可络组、脑还丹低剂量组和脑还丹高剂量组,每组10只。模型组予以生理盐水灌胃,其余各组予以相应药物治疗。治疗105d后,采用跳台实验及Y型迷宫实验进行小鼠行为学测试,并检测小鼠血清超氧化物歧化酶(superoxidedismutase,SOD)、丙二醛(malondialdehyde,MDA)、谷胱甘肽过氧化物酶(glutathioneperoxidase,GSH-Px)的含量。采用逆转录聚合酶链反应(reversetranscription-polymerasechainreaction,RT-PCR)检测小鼠大脑皮质及海马凋亡抑制基因bcl-xlmRNA的表达。结果:银可络组和脑还丹低、高剂量组小鼠的学习、记忆成绩均明显优于模型组(P<0.05或P<0.01),其中以脑还丹高剂量组的疗效最为显著(P<0.01)。银可络组和脑还丹低、高剂量组小鼠血清SOD、GSH-Px的含量及大脑皮质和海马bcl-xlmRNA的表达,均明显高于模型组(P<0.05或P<0.01);而血清MDA的含量则明显低于模型组(P<0.01)。结论:脑还丹能改善快速老化小鼠的学习、记忆能力,具有抗氧化及促进bcl-xlmRNA表达的作用。

Objective： To investigate the effects of Naohuandan Recipe on learning and memory abilities of SAM-P/8 mice and its role in anti-oxidation and anti-apoptosis. Methods： Forty SAM-P/8 mice were randomly divided into four groups, which were untreated （normal saline-treated） group, Yinkeluo Tablets （extracts of gingko leaf）-treated group, low-dose Naohuandan Recipe-treated group and high-dose Naohuandan Recipetreated group. Mice in these groups were given corresponding drugs orally for 105 days. Then the performances of learning and memory of mice were tested by a step-down passive avoidance task and a Y-maze test. The serum levels of superoxide dismutase （SOD）, malondialdehyde （MDA） and glutathione peroxidase （GSH-Px） were detected. The expression level of bcl-xl mRNA in cerebral cortex and hippocampus of mice was detected by reverse transcription-polymerase chain reaction （RT-PCR）. Results： The performances of learning and memory in the Yinkeluo Tablets-treated group, low- and high-dose Naohuandan Recipe-treated groups were significantly improved as compared with those in the untreated group （P〈0.05 or P〈0.01）, and such performance was the best in the high-dose Naohuandan Recipe-treated group among these four groups （P〈0.01）. The serum levels of SOD and GSH-Px and the expression of bcl-xl mRNA in cerebral cortex and hippocampus of mice in the Yinkeluo Tablets-treated group, low- and high-dose Naohuandan Recipe-treated groups were also significantly higher than those in the untreated group （ P 〈 0. 05 or P〈0.01）, while the serum level of MDA in the untreated group was higher than that in the other three groups （P〈0.01）. Conclusion： Naohuandan Recipe can improve learning and memory abilities of SAM-P/8 mice, and this effect may be related to its anti-oxidation efficacy and enhancement of expression level of bcl-xl mRNA.