小续命汤有效成分组的高通量筛选研究

High-throughput screening assay for groups of effective components extracted from Xiaoxuming Recipe

目的：应用高通量筛选技术，建立新型的中药复方活性成分和作用机制研究模式，探讨中药复方小续命汤发挥药理作用的物质基础。方法：观察中药复方小续命汤240个连续组分的抗氧化、抗过氧化氢损伤、抗谷氨酸损伤活性以及对神经细胞内钙离子的影响。结果：中药复方小续命汤多模型筛选结果显示，连续组分L1～L40和A100～A120的综合作用效果较好。因此，可将这两部分的连续组分重新组合，作为小续命汤抗脑缺血损伤的有效成分组。结论：中药复方小续命汤可通过多组分、多靶点途径发挥其药理作用。

Objective： To present a new approach for the research of effective components extracted from compounds of traditional Chinese medicine and their action mechanisms by high-throughput screening assay, Methods： We observed the anti-oxidation activities of 240 sequential components （L1-L120 and A1-A120） extracted from Xiaoxuming Recipe, the effects of these components on SH-SY5Y cells with H2O2 -induced and L-glutamic acid-induced damages and the levels of resting calcium ion in neurocytes of rats. Results： Some components （L1-L40, A100-A120） extracted from Xiaoxuming Recipe had the corresponding effects listed above, The combination of these components was regarded as the groups of effective components of Xiaoxuming Recipe in treating sequelae resulting from brain ischemia. Conclusion： Xiaoxuming Recipe has protective effect on brain-ischemia-induced damages through the actions of multiple components with multiple targets.