摘要
In order to find candidate proteins that are potentially associated with the phenotype in neonatal rat cardiomyocytes treated with phenylephrine(PE), the differential proteins expression pattern after α-adrenergic receptor stimulation in neonatal cardiomyocytes was analyzed by using a series of methods,including two dimensional polyacrylamide gel electrophoresis,PDQuest 2-DE software analysis and peptide mass fingerprint mapping based on MALDI-TOF-MS.The good two-dimensional pattern with a high resolution and reproducibility was obtained.Totally 14 differential protein spots were found. Out of these,11 proteins were preliminarily identified.Desmin,Txndc5,etc.were found to be up-regulated in PE-treated cardiomyocytes,while caspase-11, 4-hydroxyphenylpyruvic acid dioxygenase,etc.were found to be down-regulated.It suggests that the differential expression analysis of proteome may be useful to the further study of the related proteins and the molecular markers of cardiovascular diseases.
In order to find candidate proteins that are potentially associated with the phenotype in neonatal rat cardiomyocytes treated with phenylephrine（PE） , the differential proteins expression pattern after α-adrenergic receptor stimulation in neonatal cardiomyocytes was analyzed by using a series of methods, including two dimensional polyacrylamide gel electrophoresis, PDQuest 2-DE software analysis and peptide mass fingerprint mapping based on MALDI-TOF-MS. The good two-dimensional pattern with a high resolution and reproducibility was obtained. Totally 14 differential protein spots were found. Out of these, 11 proteins were preliminarily identified. Desmin, Txndc5, etc. were found to be up-regulated in PE-treated cardiomyocytes, while caspase-11,4-hydroxyphenylpyruvic acid dioxygenase, etc. were found to be down-regulated. It suggests that the differential expression analysis of proteome may be useful to the further study of the related proteins and the molecular markers of cardiovascular diseases.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2006年第1期79-81,共3页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:20273067
30400552)
中国科学院知识创新工程重要方向项目(批准号:KGCX2-SW-213-06)
国家重点基础研究发展规划项目('九七三'计划)(批准号:G2000056906)资助
