单纯疱疹Ⅰ型病毒即刻早期基因上游调控序列分析

Analysis of Herpes Simplex Virus TypeⅠ Immediate Early Gene Upstream Regulative Region

感染人体的单纯疱疹病毒(HSV)在宿主细胞中,以级联反应的方式表达病毒蛋白.作为最早表达的立即早期基因,其上游都具有相似的调控序列.通过α4基因上游转录相关元件的依次递减,以CAT作为报告基因,评估了在病毒感染细胞早期阶段中,各DNA序列对立即早期基因转录所发挥的可能作用.实验数据表明,HSV立即早期基因在细胞中的表达受到多种元件的共同调控,这些成簇分布的调控元件的排列分布将为深入了解HSV的复制周期提供有益的线索.

In the cells infected by HSV, various viral proteins are expressed in a cascade style. The expression of immediate-early （IE） gene occurred in early phase of infection is under the control of regulatory elements in upstream sequence of promoter. A series of pCAT expression vectors containing different upstream sequence fragment of α4 - gene, in which relative element is deleted orderly, is constructed and detected in CAT assay. The transcriptional ability of these DNA fragments on α4 - gene are evaluated to investigate their efficacy in early phase infection. The data collected indicated that the expression of IE gene in cells is controlled by various elements in its upstream. Topological distribution of these elements provides probably useful information for further understanding about the mechanism of virus replication.