苹果加工品种的糖积累与蔗糖代谢相关酶活性

Sugar accumulation and related enzyme activities in sugar metabolism of processing apple cultivars

以苹果加工品种瑞林、瑞丹、瑞星为试材研究了发育过程中不同品种苹果果实中糖积累和糖代谢相关酶活性的变化。结果表明,瑞林以积累果糖为主,成熟果实的果糖含量占总糖的53.58%,葡萄糖占24.85%;瑞丹以积累果糖为主、其次是蔗糖,2者分别占总糖的45.52%和35.39%;瑞星是蔗糖/果糖积累并重型品种,蔗糖含量占47.94%,果糖占总糖的46.65%。瑞星蔗糖积累早于其他品种并呈持续上升趋势,而瑞林果糖积累开始早,蔗糖积累缓慢。各品种蔗糖含量与酸性转化酶活性呈负相关,瑞星的酸性转化酶活性明显低于其他品种,而瑞林则表现出较高的酸性转化酶活性。发育后期各品种蔗糖含量升高与酶的净合成活性升高有关。

Three processing apple cuhivars （Malus domestica Borkh cv. Judeline, Judaine and Judestar） were used for studying the changes of sugar accumulation and enzyme activities involved in sucrose metabolism during fruit development. Results showed that more fructose was accumulated in Judeline fruit, accounted for 53.58% of the total sugar content in maturation while the amount of glucose was 24.85%. Judaine accumulated more fructose, accounted for 45.52% of the total sugar and next was sucrose, 35.39%. Judestar was sucrose-fructose-accumulating cultivar, containing 47.94% of sucrose and 46.65% of fructose. Judestar accumulated sucrose earlier than other cultivars, and sucrose rose continually during fruit development. While sucrose-accumulating-cultivars Judeline accumulated more fructose at the early stage of growth and sucrose was accumulated slowly. The sucrose content in all these cultivars had the negative correlation with the activities of acid invertase. The activity of acid invertase in Judestar was significantly lower than that in other cultivars, while Judeline had higher acid invertase activities. The increase in sucrose had correlation with the increase of net activities of sucrose-metabo- lizing enzymes.