转rolA、B、C基因枳橙快繁技术

In vitro propagation of rol A,B,C transgenic citrange

通过对转rol基因枳橙B、D、E系及对照的高接植株春梢茎段进行萌芽诱导、增殖及试管苗生根成苗的试验,摸索出适宜枳橙快繁各种培养基配方及培养条件。结果表明,以MS+6-BA1mg/L的培养基适合于芽萌发,以MS+6-BA0.5mg/L+NAA0.05mg/L的培养基适合于芽增殖,生根则以1/2MS+NAA0.5mg/L+0.2%活性炭的组合最佳,转基因各系生根率均在93.3%以上,显著高于对照生根率(66.7%)。快繁苗移栽成活率可达90%,目前生长良好。

In vitro propagation makes it possible to speed the multiplication of the rol A, B, C transgenic citrange plants, so it is possible to quickly evaluate this new rootstock. Using bud sticks of the transgenic clones （B, D, E） and the control citrange as explants, culture media containing various plant growth regulators were tested for bud burst, shoot multiplication and rooting. The results indicated that MS medium containing 1 mg/L 6-BA resulted in fastest bud bursting, MS added with 6- BA 0.5 mg/L＋ NAA 0.05 mg/L was the best choice for shoot multiplication, and 1/2MS supplied with NAA 0.5 mg/L and 0.2% activated charcoal showed quite suitable for rooting. The transgenic clones had significantly higher rooting percentage （93.3%） than that of the control （66.7%）.