督脉电针与夹脊电针对受损伤的大鼠脊髓背核神经元存活及其NOS表达的影响比较

A comparison of the effects of electric acupuncture stimulation between Dumai and Jiaji on the neuronal survival and expression of nitric oxide synthase at Clarke′s nucleus in injured spinal cord

目的比较督脉电针和夹脊电针对受损伤的脊髓背核神经元存活及其表达NOS的影响。方法将10只SD雌性大鼠(180—200g)分为督脉电针组和夹脊电针组。将两组动物制备全横断脊髓损伤模型,术后第5d进行电针治疗,一组行督脉电针治疗,另一组行夹脊电针治疗。电针治疗30d后,将两组动物灌注、固定和取材,冰冻切片,做NADPH-黄递酶组织化学染色检测脊髓背核神经元一氧化氮合酶(NOS)的活性。分别计数L1脊髓背核存活神经元数目及其表达NOS神经元数。结果督脉电针组L1脊髓背核存活神经元数目是218.50±7.23,表达NOS的神经元数是8.20±0.82,夹脊电针组L1脊髓背核存活神经元数目是188.60±6.48,表达NOS的神经元数是17.80±1.79。督脉电针组L1脊髓背核存活神经元数目多于夹脊电针组(P<0.05),并且督脉电针组L1脊髓背核表达NOS的神经元数明显少于夹脊电针组(P<0.05)。结论督脉电针比夹脊电针能够更好地促进受损伤的脊髓背核神经元存活以及抑制受损伤的脊髓背核神经元表达NOS。

Objective： To compare the effects of electric acupuncture （EA） stimulation between Dumai and Jiaji on the neuronal survival and expression of nitric oxide synthase（NOS） at Clarke＇s Nucleus（CN） in injured spinal cord. Method：Ten Sprague Dowley（SD） female rats（180--200g） were divided into Dumai EA group and Jiaji EA group. The spinal cord was completed transected in the two groups. At 5th day after operation, the animals of Dumai EA group were performed EA stimulation of Dumai acupoint, while the animals of Jiaji EA group were performed EA stimulation of Jiaji acupoint. At 30th day after EA stimulation, the animals of two groups were perfused and fixed. L1 spinal segment was cut coronally on a cryostat. The activity of NOS in CN neurons of spinal cord was detected by NADPH-diaphorase histochemistry. The number of surviving CN neurons and NOS-expressing neurons was counted respectively in L1 spinal segment. Result：In the Dumai EA group, the number of surviving CN neurons and NOS-expressing neurons was respectively 218.50±7.23 and 8.20±0.82 in L1 spinal segment. In the Jiaji EA group, the number of surviving CN neurons and NOS-expressing neurons was respectively 188.60±6.48 and 17.80± 1.79. The number of surviving CN neurons in L1 spinal segment in the Dumai EA group was more than that in the Jiaji EA grou（P〈0.05）, and the number of NOS-expressing neurons at Clarke＇s nucleus in L1 spinal segment in the dumai EA group was less than that in the Jiaji EA group （P〈0.05）. Conclusion：Compared with EA stimulation of Jiaji acupoint, EA stimulation of Dumai acupoint is a more effective way to promote the survival of neurons and inhibit NOS-expressing of neurons at Clarke＇s nucleus in injured spinal cord.