丹参酮ⅡA对放射性肺纤维化防治作用的实验研究

The protective effect of Tanshinone ⅡA in radiation-induced pulmonary fibrosis

目的探讨丹参酮ⅡA对分次照射放射性肺纤维化的防治效果及机制。方法雌性Wistar大鼠,6 MV X线右胸照射,3 Gy/次,5次/周,总量30 Gy。每次照射前1 h腹腔注射丹参酮ⅡA磺酸钠15 mg/kg。照射后5个月取大鼠肺组织进行HE染色、Masson染色、转化生长因子-β1(TGF-β1)免疫组化染色、羟脯氨酸含量及实时荧光定量RT-PCR检测TGF-β1 mRNA的表达变化。结果每克湿肺羟脯胺酸含量在对照组、照射组与丹参酮加照射组分别为(21.99±3.96)、(38.25±7.18)(、28.94±4.29)μg/g。照射组较对照组肺泡炎、纤维化病变加重,羟脯氨酸含量、TGF-β1蛋白及mRNA的表达升高;丹参酮加照射组较照射组肺泡炎、纤维化病变减轻,羟脯氨酸含量、TGF-β1表达下降。结论丹参酮ⅡA能增加肺组织对放射性损伤的耐受,其机制可能通过抑制TGF-β1表达,使炎症及纤维化病变减轻。

Objective To investigate the protective effect and it＇ s possible mechanism of Tanshinone ⅡA in radiation-induced pulmonary fibrosis, Methods Having the right hemithorax of female Wistar rats irradiated 30 Gy in 10 fractions within 14 days by 6 MV photons, the radiation-induced pulmonary fibrosis animal model was established, In the treatment group, sodium Tanshinone ⅡA sulfonate （ 15 mg/kg） was given by intraperitoneal injection 1 hour before each fraction of irradiation, Five months after irradiation, the difference of the histopathological changes, the hydroxyproline content and expression of TGF-β1 between the radiation alone group, tanshinone plus radiation and control group were analyzed by HE stain, Massion stain, immunohistochemical methor and reverse transcriptase polymerase chain reaction（RT-PCR） method. Results The histopathological comparison revealed the protective effect of Tanshinone ⅡA. The content of hydroxypmline was （21.99 ± 3.96）, （38.25 ±7.18）, （28.94 ± 4.29） μg/g in the control group, radiation alone group and radiation plus Tanshinone n A. The expression of TGF-β1（mRNA and protein） was reduced by Tanshinane ⅡA. Pathological changes of the pulmonary fibrosis was reduced by Tanshinone ⅡA yet. Condusiom Our study shows that Tanshinone ⅡA can inhibit radiation-induced pulmonary fibrosis, and the possible mechanism of its may be made possible through down-regulating the expression of TGF-β1 in the irritated lung tissue.