离子导入法和微量注射法建立铁致外伤后癫痫动物模型的对比研究

Comparison of iron-induced post-traumatic epilepsy animal models by pial iontophoresis of iron and cortical iron injection

目的对两种方法建立起来的铁致外伤后癫痫(PTE)动物模型的行为学和脑电图进行研究以比较这两种方法的差异及研究价值。方法采用离子导入法(PIFC)和微量注射法(CF-CI)建立铁致PTE大鼠模型,观察大鼠行为学变化,同时监测脑电图。结果PIFC建立起来的大鼠模型癫痫发作形式主要表现为咀嚼自动症,1d内发作频率逐渐降低,癫痫发作时脑电图上同步出现平均频率为9.66Hz,平均波幅为186.90μV的癫痫波。CFCI建立起的大鼠模型癫痫发作形式主要表现为翻转跳起,四肢抽搐,2周内发作频率逐渐降低,癫痫发作时脑电图上同步出现平均频率为16.01Hz,平均波幅为143.60μV的癫痫波。结论铁致PTE动物模型是一种稳定可靠的PTE动物模型。CFCI建立起来的PTE动物模型与PIFC相比,更有研究价值。

Objective To study the behavioral changes and electroencephalograms of iron-nduced rat models of post-traumatic epilepsy （PTE） established by pial iontophoresis of ferric chloride （PIFC） or cortical ferric chloride injection （CFCI） and compare the difference of these two methods. Methods PIFC and CFCI were used to establish the iron-induced rat models of PTE. The behavioral changes were observed and the epidural electroencephalograms of the PTE models were recorded. Results The two animal models both had the epileptic seizures, The epileptic seizure manifestation of the animal model established by the PIFC mainly presented automatic behavior of chewing, the epileptic seizure frequency decreased gradually in 1 day after the operation. The sharp waves with average frequency of 9.66 Hz and average amplitue of 186.90 μV were observed on the EEG when the rat suffered seizures;The epileptic seizure manifestation of the rat model established by CFCI mainly presented turnover upspring and limbs＇ convulsion, the epileptic seizure frequency decreased gradually in two weeks after the operation. The spike waves with average frequency of 16.01 Hz and average amplitude of 143.60 μV were observed on the EEG when the rats suffered seizures. Conclusion Iron-induced PTE animal model is stable and credible. The method of CECI is more preferable.