中国南海海绵提取物renierol对黄嘌呤氧化酶的抑制作用

Inhibition of xathine oxidase by renierol extracted from South China Sea sponge

目的:本实验的目的是探讨中国南海海绵提取物renierol对黄嘌呤氧化酶的抑制作用及对小鼠高尿酸血症的影响。方法:从海绵中分离提取renierol成分,将20、40、60μg/ml renierol或1μg/ml阳性对照别嘌呤醇(1μg/ml),分别加入黄嘌呤溶液(50μmol/ml)和0.1 U/ml黄嘌呤氧化酶中,利用5 min超氧离子生成量来测定黄嘌呤氧化酶活性(NBT显色法)。再将20、40、60μg/ml renierol或100 U/ml阳性对照SOD加入25℃预热过的邻苯三酚,在波长420 nm处测定吸光值,观察renierol对自由基的直接清除作用。建立尿酸酶抑制剂氧嗪酸钾造成小鼠高尿酸血症模型,并给高、中、低剂量renierol组小鼠口服10、203、0 mg.kg-1renierol,别嘌呤醇组给药量为2 mg.kg-1,用全自动生化分析仪测定实验鼠的血清尿酸值,观察renierol体内降尿酸作用。结果:Renierol体外是黄嘌呤氧化酶的竞争性抑制剂,其IC50为1.36μg.ml-1,在体内也有明显的降尿酸作用。结论:Renierol通过抑制黄嘌呤氧化酶来降血尿酸。

Objective：To study the inhibition of xanthine oxidase by renierol extracted from South China Sea sponge and study the influence of renierol on hyperuricemia in mice. Methods： After extracted from South China Sea sponge, renierol （20, 40, 60 μg/ml） was added to a system containing xanthine oxidase （0. 1 μ/ml） and xanthine （50 μmol/ml）; allopurinol （1 μg/ml）was also addded to the system as positive control. The5 min-fomation of superoxide anions was used to determine the activity of xanthine oxidase （Nitro Blue Btetrazolium reduction）. Renierol （20, 40, 60 μg/ml） was added to 25℃ pre heated pyrogallol autoxidation to observe its eliminating effect on free radicals through determining the absorbance rate at 420 nm wavelength. Potassium oxonate, a uricaseinhibitor, was used to induce hyperuricemia in mice and the mice were then treated withoral renierol （10, 20, 30 mg·kg^-1） orallopurinol （2 mg/kg） as positive control. The decrease of serum uric acid induced byrenierol was determined by automatic biochemical analyzer. Results： Renierol was demonstrated to be a competitive inhibitor of xanthine oxidase in vitru, with its IC〉 value being 1.36μg·ml^-1. It also decreased uric acid in vivo. Conclusion： Renierol can decrease serum uric acid through inhibiting the xanthine oxidase .