醋酸甲羟孕酮对人结肠癌细胞增殖和凋亡的影响

Effect of medroxyprogesterone acetate on proliferation and apoptosis of human colorectal cancer cells

目的:检测醋酸甲羟孕酮(MPA)对人结肠癌细胞LS174T增殖和凋亡的影响并初步分析其机制。方法:人结肠癌细胞LS174T体外培养,以不同浓度的MPA(12.5、25、50和100μmol/L)进行干预120 h,MTT法测定其对细胞增殖的抑制作用,流式细胞仪分析细胞周期、凋亡率和细胞表面Fas表达。结果:不同浓度MPA对结肠癌细胞具有显著的抑制作用。MPA处理后阻止G1期细胞向S期的进程,S期和G2/M期细胞相对减少。LS174T细胞的自然凋亡率为4.8%,12.5μmol/L组细胞凋亡率为15.1%,25μmol/L组细胞凋亡率为25.6%;MPA作用后结肠癌细胞表面Fas表达显著增强。结论:MPA对结肠癌细胞具有显著的抑制作用和诱导凋亡的作用,其机制可能与MPA使结肠癌细胞停滞于G1期及细胞表面Fas表达上调有关。

Objective：To investigate the effects of medroxyprogesterone acetate （MPA） on the proliferation and apoptosis of human colorectal cancer cells. Methods： Human colorectal cancer cell line LS174T was treated with different concentrations of MPA（12.5,25,50 and 100 μmol/L） for 120 h. The inhibitory effect of MPA was measured with MTTmethod; the cell cycle, cell apoptosis,and cell surface Fas expression were analyzed with flow cytometry. Results： MPA had significant inhibitory effect on the growth of LS174T human colon cancer cells. Cell growth was found to be arrested in Gl phase and ceils in S phase and G2/M phase were decreased. The natural apoptosis rate and the apoptosis rates at MPA 12.5 and 25 μmol/L were 4.8 %,15. 1% and 25.6% ,respectively. Cell surface Fas expression in cancer cells increased after treated with MPA. Conclusion： MPA has significam inhibitory effect on the growtb of human colon cancer cells, which may be related to Fas expression upregulation on cancer cell surface and G1 phase arrest.