肝硬化大鼠肠道Cajal间质细胞的研究

Study of Cajal interstitial cells in intestine of rats with liver cirrhosis

目的:观察肝硬化大鼠肠道Cajal间质细胞的改变,探讨肝硬化胃肠动力障碍的相关机制。方法:Wistar大鼠20只随机分为肝硬化模型组和对照组,每组10只,采用CCl4溶剂大腿根部皮下注射法制作肝硬化模型,用葡聚糖蓝-2000为胃肠内标记物,观察大鼠肠道传输,免疫组化染色观察空肠c-kit阳性Cajal间质细胞的变化,并用透射电镜观察Cajal间质细胞超微结构的改变。结果:与对照组比较,肝硬化组大鼠小肠动力明显减弱(P<0.01),空肠c-kit阳性Cajal间质细胞减少(P<0.01),透射电镜观察显示Cajal间质细胞的超微结构发生明显改变。结论:肝硬化大鼠肠道传输减弱与肠道Cajal间质细胞减少及其超微结构改变有关。

Objective： To observe the changes of Cajai interstitial celis（CIC） in the intestine of rats with liver cirrhosis, so as to study the mechanism of gastrointestinal dysfunction. Methods： Twenty Wistar rats were equally randomized into control group and cirrhosis model group. Cirrhosis model was established by CCl4, in rats. The intestinal motility changes were assayed using Dextran blue-2000 as an indicator. Immunohistochemicai staining was used to investigate the distribution of c kit positive CIC in jejunum of rats. Meanwhile, the ultrastructure of CIC was observed by electron microscope. Results： Compared with control group, the intestinal motility and the c kit positive CIC in iejunum were both markedly decreased in model group（both P〈0.01 ）. The CIC, with less organelies and dissovled cytoplasm, had decreased connections with other cells and damaged structure. Conclusion： The intestinal motility of rats with liver cirrhosis is significantly decreased,which may be associated with the changes of CIC number and uitrastructure in intestine.