黄芪多糖对人工感染IBDV雏鸡红细胞免疫功能的影响

Effect of astragalus polysaccharides on immune function of erythrocytes of chickens infected with IBDV

将由未免疫接种腔上囊病疫苗鸡的种蛋孵化而来的160只1日龄海兰白雏鸡随机分为A、B、C和D共4组。A组为对照组，B、C和D组于26日龄时按每只0．3mL的剂量用IBDV攻毒。A、B组未用黄芪多糖（APS）处理，C、D组从攻毒当日起连续胸肌注射APS6d，剂量分别为每只鸡5mg和10mg。分别在21、29、32、35、38日龄时心脏采血1～3mL，测定E—C3bRR、E—ICRR、ERER和ERIR。结果显示，雏鸡感染IBDV后可使E-C3bRR和ERER显著降低（P〈0．01）；APS处理组E-C3bRR、E-ICRR、ERER均高于A、B组（P〈0．01），其中D组最高，而ERIR则与A组相似。证实，雏鸡感染IBDV后红细胞免疫功能低下，而APS可显著提高其红细胞免疫功能。

One hundred and sixty Haline white chickens,aged 26 days-old which were negative to the antibody to infectious bursal disease virus （IBDV）, were divided randomly into four groups of A,B,C and D with 40 chickens in each group. Chickens in Group B, C and D were infected with 0.3 mL of IBDV by nasal drip and eye droppings when they were 26 days-old. Chickens in Group A and B were not injected with astragalus polysaccharide （APS）. Each chicken in Group C and D was injected with 5 mg and 10 mg of APS per day from days 26 to 31, respectively. 1-3 mL blood were taken from hearts of chickens on days 21, 29, 32, 35 and 38, respectively, and erythrocyte-C3b receptor rosette rates（E-C3bRR）, erythrocyteC3b immune complex rosette rates （E-ICRR）, erythrocyte rosette forming enhancing rates （ERER） and erythrocyte rosette forming inhibitory rates（ERIR） were examined. E-C3bRR and ERER declined significantly in the chickens infected with IBDV（P〈0.01）,E-C3bRR, E-ICRR and ERER in Groups C and D treated with APS were higher than that in Group A and B（P〈0.01）, and the ERIR in Groups C and D were similar to that in Group A. The results suggested that immunological function of chicken erythrocytes decline after infection with IBDV and APS enhances obviously the immunological functions of chicken erythrocytes.