特应性皮炎皮损角质形成细胞TLR2和TLR4的表达

TLR2 and TLR4 expression of keratinocytes in atopic dermatitis lesion

目的:探讨Toll样受体(TLR)2和TLR4在特应性皮炎(AD)表达及发病机制中的意义。方法:采用免疫组织化学法检测9例中至重度AD患者急性发作期炎性皮损TLR2和TLR4表达。结果:免疫组化检测显示在正常皮肤基底细胞层角质形成细胞(KC)胞膜散在表达TLR2和TLR4。TLR2和TLR4均在AD急性发作期炎性皮损KC胞膜及胞浆过度表达,以膜表达为主,主要定位于基底细胞层至棘细胞层。结论:AD皮损角质形成细胞TLR2和TLR4过度表达可能与AD皮肤天然免疫炎症反应有关。

Objective： To investgate the role of Toll - like receptor （TLR） 2 and TLR 4 in pathogenesis of atopic dermatitis （AD）. Methods： Immunohistochemistry was employed to study the expression of TLR2 and TLR4 in normal skin and AD skin lesions. Results： The basal keratinocytes in normal constitutively expressed TLR2 and TLR4. In contrast, in lesional epidermis from 9 patients with acute AD, TLR2 was overexpressed on the whole epidermis keratinocytes with membranous and cytoplasmic staining pattern. TLR4 was overexpressed on the whole epidermis keratinocytes with membranous staining pattern. Conclusion： These data suggest that TLR2 and TLR4 expressed by epidermal keratinocytes constitute a part of the innate immune of the skin, and increased TLR2 and TLR4 expression may be related to the skin innate immuno- inflammatory response in atopic dermatitis.