华北地区油松种群遗传多样性分析

Genetic diversity of Pinus tabulaeformis populations in North China

用酸性聚丙烯酰胺凝胶（APAGE）技术分析了华北地区5个油松种群在醇溶蛋白水平上的遗传多样性。95份材料分离出12条带，其中11条具有多态性，得到23种不同的带型。种群多态位点比例在16．67％～66．67％之间。Shannon信息指数和Nei’s指数的统计结果表明：关帝山油松种群的遗传多样性高于其它种群；华北地区油松种内遗传多样性为0．2489；种群内的遗传多样性为0．1507，占总变异的60、55％；种群间的遗传多样性为0.0982，占总变异的39．45％。

Genetic diversity of prolamins in 5 populations of Pinus tabulaeformis in North China was studied by using acidic polyacrylamide gel electrophoresis （APAGE） technique. 12 bands were identified from 95 samples on electrophoretic gel, 11 bands of them were polymorphic, and 23 band types were obtained. The percentage of polymorphic loci ranged from 16.67 % to 66.67%. The information index of Shannon and the gene diversity of Nei indicated that genetic diversity of Guandi population was higher than those of the others. The total genetic diversity of the species was 0. 248 9, and the intrapopulation and interpopulation genetic diversities were 0. 150 7 （60.55%） and 0. 098 2 （39.45%）, respectively.