七种细胞角蛋白在皮肤上皮性肿瘤中的表达

The expression of seven cytokeratins in skin epithelial tumors

目的观察7种细胞角蛋白在皮肤上皮性肿瘤中的表达,并探讨其意义。方法应用免疫组化S-P法对54例不同的皮肤上皮性肿瘤和20例正常皮肤进行细胞角蛋白7(K72.2)、细胞角蛋白8(C-51)、细胞角蛋白10(DE-K10)、细胞角蛋白14(LL002)、细胞角蛋白17(E3)、细胞角蛋白18(DC10)、细胞角蛋白19(KS19.1)标记,观察不同细胞角蛋白的表达。结果54例皮肤上皮性肿瘤包括,鳞状细胞癌10例、基底细胞癌10例、毛发肿瘤19例、皮脂腺癌2例、汗腺肿瘤13例。皮肤上皮性肿瘤中7种细胞角蛋白的表达和分布有所不同。鳞状细胞癌、基底细胞癌和毛发分化的肿瘤中细胞角蛋白多数呈弥漫表达;而汗腺分化的肿瘤中,不同部位表达不同细胞角蛋白,每种肿瘤各有特点。结论选择地检测一组细胞角蛋白的联合表达,有助于皮肤上皮性肿瘤的诊断和鉴别诊断。

Objective To observe the expression of 7 cytokeratins in skin epithelial tumors and to assess their diagnostic value. Methods The expression of 7 cytokeratins, including cytokeratin 7 （ K72.2 ）, cytokeratin 8 （ C-51 ）, cytokeratin 10 （ DE-K10 ）, cytokeratin 14 （ LL002 ）, cytokeratin 17 （ E3 ）, cytokeratin 18 （ DC10 ）and cytokeratin 19 （ KS19.1 ）, was assessed by immunohistochemical staining （S-P method） in 54 cases of different skin epithelial tumors and 20 normal skin specimens. Results Of 54 cases studied, 10 were squmous cell carcinoma （ SCC ）, 10 basal cell carcinoma （ BCC ）, 19 hair follicle tumor, 2 sebaceous carcinoma, and 13 sweat gland tumor. The expression patterns and distribution of the 7 cytokeratins were different in different skin epithelial tumors. Most of the tumor cells stained diffusely for cytokeratins in SCC, BCC and hair follicle tumor; different cytokeratins expressed in different parts of each of the subtypes of sweat gland tumors. Conclusion Analysis of a selected group of cytokeratins may be helpful in the diagnosis and differential diagnosis of SCC, basal cell carcinoma and skin adenexal tumor.