乙醇毒染大鼠睾丸总抗氧化能力、一氧化氮和一氧化氮合酶的变化与生殖细胞凋亡的关系

Relationship Between Germ Cells Apoptosis and Alterations of Total Antioxidant Capacity, Nitric Oxide Level and Nitric Oxide Synthase Activity in Testes of Rats Submitted to Alcohol Drinking

目的:探讨饮酒大鼠睾丸总抗氧化能力(T-AOC)、一氧化氮(NO)含量和一氧化氮合酶(NOS)活性的变化与生殖细胞凋亡的关系。方法:20只成年健康SD雄性大鼠随机均分为对照组和实验组,实验组和对照组分别用50%的乙醇溶液和蒸馏水按10 m l/kg每晚灌胃1次连续26 d(两个生精周期)后,用硝酸还原酶法测定睾丸组织中NO含量;用化学比色法测定其T-AOC和NOS活性;原位缺口末端标记(TUNEL)法检测生殖细胞凋亡指数(AI)的变化。结果:与对照组相比,实验组生殖细胞AI增加(P<0.01);睾丸组织T-AOC极显著下降(P<0.01),而NO含量明显上升(P<0.01)、NOS活性显著增强(P<0.01)。结论:大量饮酒能诱导生殖细胞凋亡增加,NOS活性增强导致NO的过量产生及T-AOC的显著下降是其重要原因。

Objective： To investigate the relationship between germ cells apoptosis and alterations of total antioxidant capacity （TAOC ）, nitric oxide（NO） level and nitric oxide synthase（NOS） activity in the testes of rats submitted to alcohol drinking. Methods： Twenty healthy male Sprague-Dawley rats（ 3 months old） were randomly divided into two groups： control group and experimental group. 50% alcohol and distilled water were administered intragastrically at a dose of 10 mL/kg body weight to two groups of rats respectively. After twenty-six days, the biochemical parameters（T-AOC, NO level and NOS activity） were measured with spectrophotometric determination. The TdT-mediated dUTP-X nick end labeling（TUNEL） technique was used to detect germ ceils apoptotic index（AI）. Resuits： Compared with the control group, AI was significantly higher（ P 〈 0.01 ） in the experimental group; T-AOC level reduced obviously （ P 〈 0.01 ）, but NO level and NOS activity increased predominantly （ P 〈 0.01 ）. Conclusion ： The excessive production of NO caused by the increasing of NOS activity and the decreasing of T-AOC may be the main causes that alcohol overtaking induces germ cells apoptosis.