大鼠心因性勃起功能障碍模型制作

Establishment of Rat Model of Psychical Erectile Dysfunction

目的:建立心因性勃起功能障碍(ED)动物模型。方法:获得性交配经验的36只SD雄性大鼠随机分为正常组(n=12)、模型组(n=12)及去势组(n=12)。模型组大鼠每日悬空倒吊在水面上,并不断激惹。2周后观测与雌性大鼠合笼后的性活动状况,包括雄鼠的骑乘潜伏期和骑乘次数,插入潜伏期和插入次数以及雄鼠的射精潜伏期,并计数发生性活动的大鼠数,同时取大鼠血液作血液流变学指标检测。结果:与正常组大鼠相比,模型组大鼠骑乘潜伏期[(152.5±24.6)svs(42.4±9.6)s]、插入潜伏期[(437.0±67.7)svs(130.8±39.1)s]明显延长(P<0.01),射精潜伏期[(385.3±80.0)svs(547.3±69.4)s]明显缩短(P<0.05),骑乘次数[(38.3±6.1)次vs(38.5±5.4)次]无明显差别,插入次数[(9.2±1.7)次vs(20.3±3.1)次]明显减少(P<0.01),性活动的发生率(骑乘发生率为58.3%,插入发生率为33.3%,射精发生率为16.7%)明显降低(P<0.01);但模型大鼠与去势大鼠间的性活动能力无明显差别。模型组大鼠血液粘度、红细胞比容以及红细胞聚集指数均明显高于正常组及去势组大鼠(P<0.05),而正常组与去势组大鼠间无明显差异。结论:重复悬吊加激惹方法可引起大鼠的性动机和性活动能力明显减弱,显示运用此方法可制作理想的心因性ED大鼠模型。

Objective ： To set up a method of establishing the animal model of psychical erectile dysfunction with emotional stress. Methods： All thirty-six male rats with normal sexual function were divided into three groups, i.e. normal group, model group and demasculinized group randomly according to their weights. The rats in the model group were suspended upside down in midair over the water and irritated repeatedly. Two weeks later, the sexual abilities of all rats, i.e. the times of mounting and intromitting the estrus female rats, the latent period of mounting, intromission and ejaculation, were recorded, and the number of rats that had sexual activities was also counted. And the hemorheology indices of the rats were measured. Results ： Compared with the normal rats, the latency of mounting [ （ 152.5 ± 24.6 ） s vs （42.4 ± 9.6） s ] and intromission [ （ 437.0 ± 67.7 ） s vs （ 130.8 ± 39.1 ） s ] of the model rats were longer （ P 〈 0.01 ） , but the latency of ejaculation [ （ 385.3 ± 80.0） s vs （547.3 ±69.4 ） s ] was shorter （ P 〈 0.05 ） than that of the normal. There was no significant difference in the times of mounting between normal [ （ 38.3 ± 6.1 ） vices and model rats （ 38.5 ± 5.4） vices], but the intromission times of model rats [ （9.2 ± 1.7） vices] was lower than that of the normal rats[ （20.3 ± 3.1 ） vices] ,P 〈 0.01. Compared with the normal rats, the sexual activity incidence of the model rats （mounting： 58.3% , intromission： 33.3%, ejaculation： 16.7% ） was significant lower than that of the normal rats（ 100% ） （ P 〈 0.01 ）. But there was no significant difference in the sexual ability between the model and the demasculinized rats （ P 〉 0.05 ）. The hemorheology indices, e.g. blood viscosity, hematocrit （Hct） and red cell aggregation （ RCA）, of the model rats was significant higher than that of the normal and demasculinized rats （P 〈 0.05 ）, but there was no significant difference between the normal and demasculinized rats. Conclusion： The rat model of psychical erectile dysfunction can be made ideally with psychical stress.