利用两个测序水稻品种构建微卫星连锁图谱(英文)

Construction of a Microsatellite Linkage Map with Two Sequenced Rice Varieties

利用已完成基因组测序的两个水稻品种日本晴和 9311 的数据库成功开发出水稻微卫星新标记,并利用由 90 个单株组成的日本晴 9311 F2作图群体,构建了一张包含 152 个 SSR 标记位点、覆盖基因组总长度 2 455.7 cM 的连锁图谱,有 46 个 SSR 新标记为自主开发,该图谱标记间的平均遗传距离为 16.16 cM;并将未能在 Temnykh 等人(2001)构建的图谱上定位的微卫星标记 RM345 和 RM494 定位在第 6 染色体上。通过与 Temnykh 等人(2001)和兰涛等人(2003)所构建的图谱从作图群体的类型和大小、标记的类型和数量、标记在染色体上的线性排列顺序等几个方面进行比较,所绘制的图谱其标记在染色体线性排列上与 Temnykh 等人绘制的图谱具有很高的一致性,达 93.81%。

Based on the successful development of new microsatellite markers from the data of two whole-sequenced rice varieties, japonica variety Nipponbare and indica variety 9311, an F2 population of 90 lines, which was derived from a single cross between Nipponbare and 9311, was applied to construct a genetic linkage framework map. The map covered 2 455.7 cM of total genomic length, and consisted of 152 simple sequence repeats （SSRs） loci including 46 pairs of new SSR primers developed by our research institute. The average genetic distance between two markers was 16.16 cM. In addition, markers RM345 and RM494, which have not been mapped on the Temnykh＇s map et al. （2001） were anchored on the sixth chromosome of this map. We compared this research with maps of Temnykh et al.（2001） and LAN et al. （2003） regarding the aspects of type and size of population, type and quantity of markers, and the marker arrangement order on chromosome, etc. Results indicated that the similarity of marker linear alignment was 93.81% between this map and T-map. Finally, the important significance of using sequenced rice varieties to construct linkage map was also discussed.