摘要
甘蓝型黄籽油菜具有低纤维、高蛋白及高含油量的优点, 因而已成为广大油菜育种工作者研究的重点之一。利用甘蓝型黑籽品系油研 2 号作父本,甘蓝型黄籽品系 GH06为母本,获得 132 个单株的 F2群体;以 AFLP 和 SSR 为主要分析方法,构建了包括 164 个标记的甘蓝型油菜遗传连锁图谱,其中包括 125 个 AFLP 标记、37 个 SSR 标记及一个 RAPD 和一个 SCAR 标记,分布在 19 个连锁群上,覆盖油菜基因组 2 549.8 cM,标记间平均距离 15.55 cM。利用多区间作图法,对种皮色泽 QTL 进行分析,在第 5 及第 19 连锁群上各检测到一个 QTL 位点,分别解释表型变异 46%及 30.9%。
The development of yellow-seeded varieties of Brassica napus for improving the oilseed quality characteristics of lower fiber content and higher protein and oil content has been a major focus of breeding researches worldwide in recent years. With the black-seeded ‘Youyan 2＇ as male and the yellow-seeded GH06 as female parents respectively, F2 population of 132 individuals were obtained. A linkage map was constructed with 164 markers including 125 AFLP, 37 SSR, 1 RAPD and 1 SCAR markers distributed over 19 linkage groups covering approximately 2 549.8 cM with an average spacing of 15.55 cM. Two loci located on the 5th and 19th group were detected for the trait of seed coat color based on the linkage group using multiple interval mapping method and explained 46% and 30.9% of the phenotypic variation, respectively.
基金
This work was supported by the National High Technology Research and Development Program (863 Program) and Open Project for Visiting Scholar in National Key Laboratory of Crop Genetic Improvement.
