IGF-1对小鼠牙囊细胞生物学特性的影响

Effects of IGF-1 on the biological characteristics of mouse dental follicle cells

目的：探讨胰岛素样生长因子-1（IGF-1）对小鼠牙囊细胞增殖、蛋白含量及分化能力影响。方法：将第3代小鼠牙囊细胞与不同浓度IGF-1（0．005、0．01、0．05、0．1、0．5mg／L）共孵育后，测定IGF-1对细胞增殖、碱性磷酸酶活性、总蛋白含量的影响；并用VonKossa法检测0．05和0．1mg／L的IGF-1对牙囊细胞矿化结节形成能力的影响。结果：在0．05～0．1mg／L的浓度范围内，IGF．1能显著促进牙囊细胞的增殖，使ALP活性及总蛋白含量增加（P〈0．01），但三者的最佳效应浓度有所差异，当浓度升高至0．5mg／L时，促进效应下降，与对照组无显著性差异（P〉0．05）；0．05和0．1mg／L的IGF-1均能显著促进牙囊细胞矿化结节的形成（P〈0．05，与对照组相比）。结论：合适浓度的IGF-1能促进牙囊细胞的增殖，增加蛋白含量及向成骨细胞（成牙骨质细胞）方向分化的能力。

Objective：To study the effects of IGF-1 on the proliferation, total protein amount and cytodifferentiation of mouse dental follicle cells. Methods ： The dental follicle cells of passage 3 were incubated with different concentrations（0. 005, 0. 01,0.05,0.1 and 0. 5 mg/L） of IGF-1 respectively, cell proliferation, alkaline phosphatase （ ALP）, total protein amount were measured by MTY assay and spectrophotometer respectively. Effects of O. 05 and O. I mg/L IGF-1 on mineralization potential were studied by Von Kossa staining. Results：IGF-1 （ml/L） at 0. 005-0.1 increased the proliferation and total protein amount（P 〈 0. 05 or P 〈 0. 01 ）, at 0. 01 -0. 1 increased ALP expression（ P 〈 0. 01 ）, at 0. 05 and 0. 1 increased the mineral noclule formation （ P 〈 0. 05 ） of the cells. Conclusion：Proper concentrations of IGF-1 may promote dental follicle cell proliferation, protein synthesis and differentiation towards osteoblasts（cementoblasts）.