DPC4基因在非小细胞肺癌中的表达及其与微血管形成的关系

Expression of DPC4 Gene in Non-small Cell Lung Carcinoma and Its Relation to Angiogenesis

目的:研究DPC4(deleted in pancreatic carcinoma locus 4,DPC4)基因在非小细胞肺癌NSCLC中的表达及其与血管内皮生长因子(VEGF)表达和肿瘤内微血管密度(MVD)的关系。方法:利用免疫组织化学SP法检测52例NSCLC组织、19例相应的癌旁正常肺组织中DPC4、VEGF的表达和MVD值。结果:DPC4在肺癌原发灶中的阳性表达率为63.5%(33/52),与癌旁正常肺组织中的阳性表达率89.5%(17/19)相比,DPC4阳性表达水平显著降低,差别有显著性意义(P<0.05);DPC4与组织学类型、肿瘤细胞分化程度无关(P>0.05),但与淋巴结转移显著相关(P<0.05)。52例NSCLC中,DPC4的表达与VEGF、MVD值均呈负相关。结论:DPC4的低表达可能是肺癌发生过程的早期事件,并可通过直接或间接的作用促进肺癌血管生成,从而促进肺癌的淋巴结转移。

Objective： To study the expression of DPC4 （deleted in pancreatic carcinoma locus 4, DPC4） gene in non-small cell lung carcinoma （NSCLC as well as its association with angiogenesis. Methods： The expression of DPC4, VEGF and CD34 was detected in 52 cases with primary NSCLC and 19 adjacent normal lung tissues by using immunohistochemical SP methods. Results： Positive expression rate of DPC4 in primary lung cancer tissues was 63. 5% （33/52）,compared with the positive rate 89.5% （17/19） in adjacent normal lung tissues. And the DPC4 expression level apparently degraded compared with the normal tissues（P〈0.05）. The positive expression of DPC4 had no correlation with tissue type and cellular differentiation（P〉0.05）, but it was closely associated with lymph node metastasis（P〈0.05）. In 52 patients with NSCLC, the relationship between DPC4 and VEGF, and between correlation（r=-0. 303, P=0. 020）. Conclusion： DPC4 and MVD showed apparently negative The low expression of DPC4 could be an early event during the course of the NSCLC＇s formation. Simultaneously,it also promoted lymph node metastasis by promoting the angiogenesis in NSCLC directly or indirectly.