长效醋酸甲孕酮降低大鼠子宫内膜异位症模型中内膜RANTES的表达

Down-regulation Effect of DMPA on the Expression of RANTES in Experimental Endometriosis Rats

目的：探讨长效醋酸甲孕酮(DMPA)对大鼠实验性子宫内膜异位症模型中正常T细胞表达和分泌的受激活调节因子RANTES表达的影响。方法：参照Jones方法建立大鼠子宫内膜异位症动物模型,将建模成功的大鼠随机分为对照组和DMPA治疗组,采用EI．ISA法检测两组子宫在位内膜、异位内膜和血清中RANTES的水平。结果：DMPA治疗组在位、异位内膜和血清中RANTES水平(中位数)分别为89．2,199．4,6 871．5 ng／L;对照组在位、异位内膜和血清中RANTES水平(中位数)分别为141．8,401．8,7 523．3 ng／L。DMPA治疗组在位内膜RAN—TES的水平与对照组比较,差异有显著性(P％0．05)；其异位内膜RANTES的水平与对照组比较,差异有极显著性(P<0．01)；两组血清中的RANTES水平差异无显著性(P>0．05)。结论：DMPA能有效降低子宫内膜异位症模型中内膜RANTES的表达。DMPA可能通过下调内膜RANTES的表达,抑制盆腔的炎性反应,从而缓解子宫内膜异位症患者的盆腔疼痛。

Objective： To investigate effects of DMPA （Depot medroxyprogesterone acetate） on the expression of RANTES （regulated on activation, normal T cell expressed and secreted） in experimental endometriosis models of rats. Methods： Jones＇ method was used to develop an animal model of endometriosis in rats. Successful models were randomized into the control group and the DMPA-treated group. RANTES concentrations in eutopic and ectopic endometrium and serum were determined by ELISA. Results： The levels of RANTES in eutopic and ectopic endometrium and serum from DMPA treated rats were 89.2, 199.4 and 6 871.5 ng/L respectively （median）. In the control group, the concentrations of RANTES in eutopic and ectopic endometrium and serum were 141.8,401.8 and 7 523.3 ng/L respectively （median）. The difference of RANTES, between the treatment group and the control group, either in eutopic or in ectopic endometrium, was significant （P〈0.05 and P〈0.01, respectively）. There was no statistic difference between that in the two groups in serum（P〉0.05）. Conclusion： These studies indicate that DMPA downregulates endometrial RANTES expression in experimental endometriosis models. The clinical effectiveness of DMPA treatment in endometriosis-associated pelvic pain may be attributed to its inhibition of RANTES production and its suppression of inflammatory responses in the pelvis.