喉鳞状细胞癌中PUMA、P53基因的表达及其临床意义

Expression of PUMA and P53 in Laryngeal Squamous Cell Carcinoma and Their Clinical Significance

目的:研究PUMA、P53在喉鳞状细胞癌组织中表达,探讨PUMA在喉鳞癌发生发展中作用及与P53的关系。方法:采用免疫组织化学SP法检测40例喉癌组织以及16例癌旁组织中的PUMA和P53的表达情况。结果:PUMA在喉癌中的表达阳性率(62.5%)低于癌旁组织(87.5%),P<0.05,PUMA的表达与喉鳞癌患者的颈淋巴结转移及预后有关。P53在喉癌中的表达阳性率(65.0%)高于癌旁组织(0%),P<0.01,P53的表达与喉鳞癌患者的肿瘤分化程度、颈淋巴结转移及预后有关。PUMA与P53在喉鳞癌中的表达呈负相关关系。结论:PUMA可能是喉鳞癌的一个重要的预后标记物。P53的表达与肿瘤分化、淋巴结转移有关,可作为判断预后的一个有意义的指标。

Objective： To study the expression of PUMA and P53 in laryngeal squamous cell carcinoma （LSCC） and the relation between PUMA and P53. Methods.. The expression of PUMA and P53 protein were examined by immunohistochemical method in 40 cases of LSCC and 16 cases of paracarcinoma tissue. Results： PUMA protein positive expressive rate in the laryngeal squamous cell carcinoma （62.5%） was lower than that in the para-carcinoma tissue （87.5%）, P〈0. 05. The expression of PUMA protein was correlated with cervical lymph node mestastasis and prognosis. P53 positive expressive rate in LSCC （65.0%） was higher than that in the para-carcinoma tissue （0%）, P〈0.01. The expression of P53 protein was correlated with tumor differentiation grade, cervical lymph node mestastasis and prognosis. There was a negative relationship between the expression of PUMA and P53 in LSCC. Conclusion： The expression of PUMA may be an important prognostic marker for LSCC. The expression of P53 protein was correlated with tumor differentiation grade,cervical lymph node mestastasis and can be used as a valuable marker for the prognosis of LSCC.