盐酸阿比朵尔抗腺病毒7型的实验研究

Antiviral Effect of Arbidol Hydrochloride on Adenovirus Type 7 in Vitro

目的:明确抗病毒新药盐酸阿比朵尔体外抗腺病毒7型(ADV-7)的作用方式。方法:主要通过观察细胞病变效应(CPE)、噻唑蓝(MTT)比色法检测细胞活性、病毒滴定3种方法从药物抗病毒生物合成、药物直接杀伤病毒以及药物抗病毒吸附3方面检测盐酸阿比朵尔的作用方式。结果:盐酸阿比朵尔对ADV-7无直接杀伤作用,也不能阻止ADV-7的吸附和穿入,各用药组均出现典型的CPE,但盐酸阿比朵尔能明显抑制ADV-7的生物合成作用,对细胞的半数毒性浓度(CC50)为85.37 mg/L,对ADV-7的半数有效浓度(IC50)为15.39 mg/L,治疗指数(TI)为5.55,且随着药物浓度的增加,病毒所致的CPE效应逐渐减弱,培养液中的病毒滴度也逐渐降低,而病毒抑制率则明显升高。结论:盐酸阿比朵尔在体外通过抑制病毒生物合成而发挥抗ADV-7的作用,其具体作用机制有待进一步研究。

Objective： To investigate the antiviral activity of Arbidol hydrochloride against adenovirus type 7 （ADV-7） in vitro. Methods. The antiviral effect of Arbidol was studied by assaying infected cells for protection from cytopathic effect （CPE）, adopting MTT colorimetric assay for viral inhibitory rate and viral titers. Results. Arbidol displayed antiviral activity against ADV-7 when added after infection with 50 % cytotoxic concentration （CCs0） and 50 % inhibitory concentration （IC50） of 85.37 mg/L and 15.39 mg/L,resulting in a treatment index （TI） of 5.55. With the increase of the drug dose, the degree of CPE and viral titers decreased correspondingly, whereas viral inhibition rate increased. As typical CPE were observed at all concentrations, Arbidol showed no abilities of directly-killing ADV-7 and had no effect on viral absorption and penetration. Conclusion. These results indicate that Arbidol suppresses the late stage of viral cycle. Arbidol should be considered further as a multi-factorial and prospective antiviral substance.