草血竭抗流感病毒的研究

Antiviral Activity of Polygonum Paleaceum Wall Against Influenza Virus in Vitro

目的:为研究草血竭体外抗流感病毒作用机理。方法:采用噻唑蓝(MTT)比色法检测细胞活性及空斑减数实验测定病毒滴度,以确定草血竭两个有效成分细胞内抗流感病毒的作用。结果:实验发现草血竭水煮醇沉干浸膏和草血竭纯化部分既不能阻断流感病毒侵入细胞,也不能抑制流感病毒的生物合成,且各药物浓度组均出现典型的细胞病变效应(CPE);但草血竭水煮醇沉干浸膏与草血竭纯化部分能够明显杀灭病毒,即随药物浓度的增加,CPE特征逐渐减弱,病毒滴度也逐渐降低,而空斑减数率明显升高。结论:草血竭在体外通过直接灭活作用发挥抗流感病毒作用。

Objective： To investigate polygonum paleaceum wall＇s anti-influenza virus effects in vitro. Methods： The anti-viral effects of two active components abstracted from polygonum paleaceum wall were investigated by the technique of plaque test in culture, by applying MTT colorimetric assay for viable cell rate. Results： In vitro, two active components were observed to be inhibitors against influenza virus in a concentration-dependent manner. As the dose of drugs increased, viral titers decreased and plaque inhibition rate increased correspondingly. Conclusion： Polygonum paleaceum wall directly inactivate influenza virus but not inhibit the viral biosynthesis or block the adsorption to susceptible cells in vitro.