槲皮素与芦丁对离体大鼠主动脉环的舒张作用及机制

Comparison of vasodilatation effect between quercetin and rutin in the isolated rat thoracic aorta

目的:比较研究槲皮素与芦丁对离体大鼠胸主动脉环的作用及其可能的途径。方法:采用累积加药法,检测槲皮素和芦丁对去氧肾上腺素(pheny lephrine,PE)预收缩的胸主动脉环张力的影响。结果:槲皮素对离体大鼠内皮完整和去内皮的胸主动脉环均有浓度依赖性的舒张作用,而芦丁对PE预收缩血管的舒张作用是内皮依赖性的。槲皮素和芦丁对内皮完整的胸主动脉环的最大舒张反应分别为(77.20±6.11)%和(44.28±7.48)%,但两者对内皮完整的胸主动脉环最大舒张的半数有效浓度无明显差异。用一氧化氮合酶抑制剂L-NAM E(0.1 mm o l/L)预处理后,可阻断芦丁诱导的舒张血管作用,但不能阻断槲皮素引起的舒张血管作用;用鸟苷酸环化酶抑制剂亚甲蓝(10μm o l/L)预处理后,两者的血管舒张作用均被阻断。用环氧合酶抑制剂吲哚美辛(10μm o l/L)预处理后可减弱槲皮素诱导的舒张血管作用,但不能阻断芦丁引起的舒张血管作用。结论:槲皮素的舒血管作用强于芦丁,槲皮素可能是通过鸟苷酸环化酶和环氧合酶途径产生非内皮依赖性的血管舒张作用,而芦丁可能是通过NO-鸟苷酸环化酶途径产生内皮依赖性的血管舒张作用。

Objective： To determine the possible difference in vasodialtation effect of quercetin and rutin. Methods： The isolated rat thoracic aorta was treated with phenylephrine （PE） ,and the effects of quercetin and rutin on the preconstricted aorta rings with or without endothelium were determined by organ bath technique. Nitric oxide synthase inhibitor L-N （G）-nitroarginine methyl-ester （L-NAME）, guanylyl cyclase inhibitor methylene blue, cyclooxygenase inhibitor indomethacin were used to explore the mechanism. Results： Quercetin （10-160μmol/L） caused vasorelaxation of aorta rings preconstricted with PE in endothelium-intact and denuded aorta rings in a dose-dependent manner. Rutin（10-160μmol/L） caused dose-dependent vasorelaxation in endothelium-intact rings preconstricted with phenylephrine,but not in denuded aorta rings. The maximal response （Rmax） values calculated from vasorelaxation curves of quercetin and rutin were （77. 20± 6. 11） % and （44. 28 ±7. 48） %, respectively. There was no difference between median effective concentration （EC50） values of quercetin and rutin. Pretreatment with L-NAME （0. 1 mmol/L） abolished the vasorelaxation by rutin, but did not influence the vasodilating effect of quercetin in endothelium-intact rings. Pretreatment with methylene blue （10 mmol/L） canceled the vasorelaxation both by quercetin and rutin. Pretreatment with indomethacin （10μmol/L） attenuated the vasodilatation of quercetin, but did not affect the vascular effect of rutin. Conclusion： The vasodilatation effect of quercetin is more potent than rutin. The vasodilatation effect of quercetin might be mediated by guanylyl cyclase and cyclooxygenase-dependent pathway, while the vasodilatation by rutin might be via nitric oxideguanylyl cyclase pathway.