复合缓释微球的胶原膜促创面愈合的实验研究

PROMOTION OF WOUND HEALING THROUGH INCORPORATION OF bFGF-IMPREGNATED MICROSPHERES INTO COLLAGEN MEMBRANE

目的观察复合缓释微球的胶原膜对猪皮肤创面损伤的促愈合作用。方法将20μl的碱性成纤维细胞生长因子(basicfibroblastgrowthfactor,bFGF)溶液滴加在2mg的冻干微球上,复合于胶原膜内。取2.5～3月龄健康约克猪6只,随机分为3组,在每只猪背部制作6个4cm×4cm全层皮肤缺损创面模型。将包裹bFGF的明胶缓释微球与胶原基多孔膜复合制备,作为实验组移植创面,对照组及空白组分别给予复合bFGF的胶原膜和空白胶原膜,术后每日观察创面愈合情况,14、21、28和35d图像分析计算创面愈合率,3、7、14、21、28和35d行组织学染色,观察各组材料对猪皮肤全层缺损创面的影响。结果制备的复合微球的胶原膜内明胶微球大小均一,平均粒径为12.36±3.56μm,孔径为80～150μm。实验组、对照组及空白组创面愈合时间为27.30±1.14、29.18±1.69、31.12±1.36d,3组组间比较,差异均有统计学意义(P<0.05);实验组创面愈合率在术后14、21、28和35d均高于对照组及空白组,差异有统计学意义(P<0.05);术后各时间点组织学观察实验组愈合创面的上皮化程度均优于对照组及空白组。结论复合bFGF缓释微球的胶原膜能有效促进猪皮肤全层缺损创面的愈合,可作为具有应用前景的覆盖材料进一步研究。

Objective To investigate the promotion effects of the collagen membrane incorporating bFGF- impregnated microspheres on the wound healing of the pig skin losing its full-thickness layers. Methods The bFGF containing microspheres was added into the dry microspleres. The collagen membranes were prepared by incorporating bFGF-impregnated microspheres, and 6 York pig models of skin wounds with loss of their full-thickness layers were established for the observation of the effects on the wound healing. Results The healing time and the 28 day healing rate were 27. 30±1.14 days and 98. 12%±1. 97%, respectively. The healing rate was significantly higher and the healing time was significantly shorter in the experimental group than in the control group （P〈0.05）. The histological examination showed that the proliferation condition of the epidermis was also much better in the experimental group. Conclusion Incorporation of bFGF-impregnated microspheres into the collagen membrane is a promising method of promoting the healing of the wound with a loss of the full-thickness skin.