血啉甲醚在兔脉络膜黑色素瘤组织中的分布

Distribution of hematoporphyrin monomethyl ether in rabbit choroidal melanomas

目的 应用血管造影和组织冰冻切片荧光显微镜观察的方法，研究国产新型光敏剂血啉甲醚（hernatoporphyrin monomethyl ether，HMME）在兔脉络膜黑色素瘤眼部组织的分布。方法建立改良的兔脉络膜黑色素瘤动物模型。肿瘤生长3～4mm后进行药物分布买验。28只兔眼（肿瘤20眼，正常眼底4眼，虹膜4眼）静脉注射HMME（10mg·kg^-1）后96h内血管造影；于兔静脉注射HM（10mg·kg^-1）后的5min、15min、30min、60min、180min、480min和24h、48h、72h各取兔眼球2眼制作组织冰冻切片，荧光显微镜下观察药物在肿瘤及眼部各组织的分布，并进行半定量分析。结果于静脉注射HMME后3～4h在肿瘤组织选择性聚积达到最高。结论HMME可以在兔脉络膜黑色素瘤中选择性聚集、滞留，静脉注射后3～4h是光动力治疗的适宜时间。

ObJective To investigate the distribution of hematoporphyrin monomethyl ether（HMME） in rabbit choroidal melanomas by dye fluorescence angiography and fluorescence microscopy. Methods Pigmented choroidal turners models were established by improved methods. Digital HMME angiograms were performed in 28 rabbits （20 eyes for experimental choroidal melanomas,4 eyes for normal choroids, and 4 eyes for irides）. HMME （ 10 mg·kg^-1） was injected intravenously and images of tumor, normal choroid, or iris were obtained 5 minutes, 15 minutes, 30 minutes, 60 minutes, 180 minutes and 480 minutes, 24 hours, 48 hours and 72 hours after the injection. Fluorescence microscopy was performed in 20 rabbits to observe the distribution of HMME and the result was analyzed semi-quantitatively. Results The maximal hematoporphyrin monomethyl ether fluorescence was observed in tumorous tissues 3-4 hours after injection. Fluorescence photometry corroborated these results. Conclusion HMME is selectively accumulated in choroidal melanomas 3-4 hours after injection, which is suitable time for photodynamic therapy.