摘要
比较研究了峨眉山野生八角莲根、组培愈伤组织及其诱导根的鬼臼毒素含量,为八角莲的快速繁殖和利用生物技术生产鬼臼毒素提供科学依据.高效液相色谱技术(HPLC)显示组织培养的八角莲愈伤组织和诱导根中均含有鬼臼毒素,分别为0.117%和0.148%,野生品的根中其含量为0.600%.通过组织培养可在不破坏自然资源的前提下快速生产鬼臼毒素,从而为开发鬼臼毒素类抗癌药物提供大量原料.
In this study, HPLC was used to detect the podophyllotoxin content in three parts of Dysosma versipellis （Hance）. The content was 0.60% in the wild root, while in the calli and the induced root from this calli, they were 0. 117% and 0. 148%, respectively. The results suggest that it is possible to produce podophyllotoxin quickly by tissue culture, and provide much raw material to antitumor drugs with little damage to the natural resources.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2006年第1期56-59,共4页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
浙江省科技厅计划项目(2004C32064)
关键词
鬼臼毒素
八角莲
HPLC
组织培养
抗肿瘤作用
podophyllotoxin
Dysosma versipellis （Hance）
HPLC
tissue culture
antitumor
