摘要
The purpose of the research is to ex- plore the effect of different surface modifiers in preparation of magnetic carriers-supported cis-plat- inum (CDDP) complex and their characteristics. So- dium alginate (SA) and N-oleoylsarcosine (NOSARK) were used respectively as linkers between CDDP and magnetic nanometer particles (MNP), and carri- ers-supported CDDP complex of carboxymethyl dex- tran (CM-dex) was used as the control. The physical and chemical characteristics of CDDP-MNP were measured by transmission electron microscope, photon correlation spectrum (PCS), spectrophotome- ter, etc. The advantages and disadvantages of the two linkers were analyzed through comparing the stability and quantity of reversibly bound CDDP. The cis-platinum magnetic nanometer particles were found to have the shape of approximately uniformly pellets under transmission electron microscope. The distribution of PCS diameter was 43―52 nm and the diameter of magnetic nucleus of CDDP- MNP com- plex was about 8.8±1.3 nm. The drug- loading ca- pacity of SA is larger than that of NOSARK, and similar to that of CM-dex. The SA-modified CDDP-MNP solution remained stable after being kept at 4℃ for 40 d. SA is superior to NOSARK as a magnetic nanoparticles surface modifier. Natural SA can supersede CM-dex to serve as linker between CDDP and MNP. In practical application, we should take both the maximal reversible CDDP-bonding capacity and CDDP utilization rate into consideration.
The purpose of the research is to explore the effect of different surface modifiers in preparation of magnetic carriers-supported cis-platinum （CDDP） complex and their characteristics. Sodium alginate （SA） and N-oleoylsarcosine （NOSARK） were used respectively as linkers between CDDP and magnetic nanometer particles （MNP）, and carriers-supported CDDP complex of carboxymethyl dextran （CM-dex） was used as the control. The physical and chemical characteristics of CDDP-MNP were measured by transmission electron microscope, photon correlation spectrum （PCS）, spectrophotometer, etc. The advantages and disadvantages of the two linkers were analyzed through comparing the stability and quantity of reversibly bound CDDP. The cis-platinum magnetic nanometer particles were found to have the shape of approximately uniformly pellets under transmission electron microscope. The distribution of PCS diameter was 43-52 nm and the diameter of magnetic nucleus of CDDP- MNP complex was about 8.8±1.3 nm. The drugloading capacity of SA is larger than that of NOSARK, and similar to that of CM-dex. The SA-modified CDDP-MNP solution remained stable after being kept at 4℃ for 40 d. SA is superior to NOSARK as a magnetic nanoparticles surface modifier. Natural SA can supersede CM-dex to serve as linker between CDDP and MNP. In practical application, we should take both the maximal reversible CDDP-bonding capacity and CDDP utilization rate into consideration.
基金
The authors thank Dr.Hong Chen for her assistance in revision of this manuscript.This work was supported by the Science and Technology Foundation of Guangdong Province(Grant No.730311)
the Natural Science Foundation of Guangdong Province(Grant No.36645)
the Science and Technology Special Foundation of Guangzhou City(Grant No.2004Z3-D2091).
关键词
顺铂
磁性纳米粒子
N-油酰基肌氨酸
海藻酸钠
超顺磁性
cis-platinum, magnetic nanometer particles, carriers, N-oleoylsarcosine, sodium alginate.
