当归多糖组分AP-3诱生小鼠脾细胞IL-2和IFN-γ的作用

Effect of Angelica sinensis polysaccharide fraction AP-3 on IL-2 and IFN-γ induction

目的研究当归多糖组分AP-3对细胞因子IL-2和IFN—γ的诱生作用，以探讨其免疫调节的特点。方法流式细胞术测定培养的脾细胞中Cd4^＋细胞比例；酶联免疫法测定培养上清液中IL-2和IFN-γ的浓度；RT—PCR法测定IL-2和IFN-γ mRNA的转录水平。结果AP-3在0．6～2μmol·L^-1，能显著提高培养脾细胞CD^4＋细胞的百分率；在2～6μmol·L^-1，AP-3时间、剂量依赖性地增加培养的细胞上清液中IL-2的浓度和细胞内IL-2 mRNA的转录水平，而对于IFN-γ和IFN-7 mRNA，则先升高后降低，并呈现剂量依赖性。结论当归多糖能够促进IL-2和IFN-γ的分泌，激活Th1细胞，从而发挥免疫调节作用。

Aim To investigate the effects of Angelica sinensis polysaccharide fraction AP-3 on IL-2 and IFN-γ induction and its further immunomodulatory feature. Methods The percentage of CD4^＋ lymphocyte was detected by flow cytometric method, the production of IL-2 and IFN-γ in cell culture supernatant were determined by ELISA, mRNA expressions of IL-2 and IFN-γ cytokines were detected by RT-PCR. Results At the range of 0. 6 -2 μmol·L^-1, AP-3 significantly enhanced the percentage of CD4^＋ lymphocytes in total splenocytes. At the range of 2 -6 μmol·L^-1, the treatment of AP-3 augmented both productions of IL-2 in cell culture supernatant and cell IL-2 mRNA transcription level in a time and dose dependent manner. While in the case of IFN-γ, AP-3 stimulated at early time after exposure but down-regulated thereafter. Conclusion Angelica sinensis polysaccharide could regulate the immune response through upregulating IL-2, IFN-γ expression and activating Th1 cell.