甘蓝型油菜华油杂6号及其亲本的基因差异表达研究

Analysis of Differential Gene Expression Pattern in Brassica napus Hybrid Huayouza6 and Its Parents Using Arabidopsis cDNA Microarray

目的探讨甘蓝型油菜在基因表达水平上的杂种优势机理,以期为最终揭示杂种优势的遗传基础积累试验数据。方法应用拟南芥cDNA芯片,以华油杂6号及其亲本为材料,分析杂种和亲本在花蕾中的基因差异表达。结果杂种和母本8086A之间,有83个上调的转录本和331个下调的转录本;杂种和父本7-5之间,有94个上调表达的转录本和423个下调表达的转录本。在上调表达的转录本中,存在较多与光合作用相关的基因,讨论了这些基因和甘蓝型油菜华油杂6号杂种优势之间的关系。磷酸核酮糖(PRK)的Northern印迹结果与芯片显示结果基本一致。结论基础代谢基因对甘蓝型油菜杂种优势的形成影响较大。拟南芥cDNA芯片可以广泛应用于甘蓝型油菜及其近源物种的基因表达分析。

[ Objective ] The purpose of this study is to probe the mechanism of heterosis of Brassica napus in gene expression level, and to accumulate the experimental data for final demonstrating the genetic basis of heterosis. [Method] Two cDNA-based Arabidopisis microarrays were used to analyze gene differential expression in bud of an elite B.napus hybrid Huayouza6 and its parents. [Result] There were 83 over-expression transcripts and 331 under-expression transcripts between Huayouza6 and its female parent 8086A and 94 over-expression transcripts and 423 under-expression transcripts were demonstrated between Huayouza6 and its male parent 7-5. Further analysis showed that there were significant number of genes responsible for photosynthesis, which its implication for heterosis was discussed. Northern analysis of phosphoribulokinase coincided with its expression pattern derived from hybridization of Arabidopsis cDNA microarray and B. napus mRNA. [Conclusion] This system of heterologous hybridization analysis should be applicable to other close relatives of Arabidopsis thaliana, and basic metabolism genes exert significant influence on heterosis of Brassica napus.