摘要
The kinds and activities of proteolytic enzymes in the digestive systems (tongue, esophagus, stomach, duodenum, liver, pancreas and large intestine) of two Amur tiger cubs (Panthera tigris altaica) were investigated using protein detection and SDS-G-PAGE. The results showed:1) the digestive system of Amur tiger cubs had 30 different kinds of proteolytic enzymes ; 2) the mostly optimal pH for these enzymes was a neutral environment, and an acid condition highly restrained their activity; 3) the proteolytic enzymes in the tongue and esophagus were fewer than in other digestive organs and their activities were weak as well; 4) the duodenum had the most kinds of proteolytic enzymes with the strongest activities under various pH conditions; 5) the 16 kD enzyme bands always existed under neutral and alkalescence pH conditions in all digestive organs except the tongue.
The kinds and activities of proteolytic enzymes in the digestive systems （ tongue, esophagus, stomach, duodenum, liver, pancreas and large intestine） of two Amur tiger cubs （ Panthera tigris altaica） were investigated using protein detection and SDS-G-PAGE. The results showed ： 1 ） the digestive system of Amur tiger cubs had 30 different kinds of proteolytic enzymes ; 2 ） the mostly optimal pH for these enzymes was a neutral environment, and an acid condition highly restrained their activity ; 3 ） the proteolytic enzymes in the tongue and esophagus were fewer than in other digestive organs and their activities were weak as well ; 4 ） the duodenum had the most kinds of proteolytic enzymes with the strongest activities under various pH conditions; 5） the 16 kD enzyme bands always existed under neutral and alkalescence pH conditions in all digestive organs except the tongue.
出处
《兽类学报》
CAS
CSCD
北大核心
2006年第1期98-100,共3页
Acta Theriologica Sinica
基金
河南省自然科学基金资助项目(0411032500)
河南省动物学省级重点学科经费资助
关键词
东北虎幼体
消化系统
蛋白水解酶
复性电泳
Digestive system
Panthera tigris altaica
Proteolytic enzymes
SDS-G-PAGE
