诃子抗CPG ODN组分的分离及活性评价

Extraction of anti-CPG ODN component from Terminaliachebula retz and their activity evaluation

目的:从诃子中分离制备具有拮抗CPG ODN作用的活性组分。方法:应用水提、离子色谱技术等方法,从诃子中分离制备具有拮抗CPG ODN活性的组分;并应用生物传感器技术,对所得到的组分进行与CPG ODN结合活性的筛选;观察其对CPG ODN刺激的小鼠RAW 264.7细胞释放炎症介质的抑制作用。结果:应用生物传感器技术筛选出与CPGODN结合活性最高的诃子作为研究对象,应用离子色谱技术从诃子中分离出3组分,其中有3个组分均具有一定的与Lipid A的结合活性,以第3组分与Lipid A的结合活性最强;并对CPG ODN刺激的小鼠RAW 264.7细胞释放TNF-α具有显著的抑制作用;结论:从诃子中分离出的第3组分具有显著的拮抗CPG ODN活性。

AIM： To extract different components from Terminaliachebula retz and evaluate their anti-CPG ODN activities. METHODS： Anti- CPG ODN fractions were extracted through water extraction and ion-exchange HPLC. Their binding activities to CPG ODN were selected through sensor technology. Their inhibiting activity to the release of TNF-α from RAW 264.7 was determined. RESULTS： Three anti-CPG ODN fractions were extracted from Terminaliachebula ret. Terminaliachebula retz 3 fraction was proved to have most powerful binding activity to CPG ODN, and inhibit the release of TNF-a of RAW 264.7. CONCLUSION： The Terminaliachebula retz 3 fraction has marked anti-CPG ODN activity.