前列腺癌中TSP-1表达与血管生成之间关系的探讨

Correlation between the Expression of Thrombospondin-1 and the Angiogenesis in Prostatic Carcinoma

目的:探讨前列腺癌中肿瘤血管生成与凝血酶敏感蛋白-1(TSP-1)表达的相关性。方法:采用免疫组织化学法检测22例前列腺癌中微血管密度(MVD)值和TSP-1的表达。结果:前列腺癌中TSP-1阳性表达率为72.7%(16/22),绝大多数表达位于肿瘤细胞的胞浆中,少数表达位于肿瘤细胞外基质。在22例前列腺癌组织中平均MVD为71.21±31.14/100倍视野,具有TSP-1强表达的肿瘤组织中显示MVD值较高,TSP-1的免疫活性与微血管密度间呈显著相关性(r=0.54,P=0.009)。结论:TSP-1在前列腺癌组织中呈高表达与前列腺癌的血管生成相关,有促进前列腺癌中血管生长的作用,并参与前列腺癌血管基因的表达。

Objective： To observe the expression of thrombospondin-1 （TSP-1） separately in prostatic carcinoma, and its relationship with the angiogenesis. Methods： The expression of TSP-1 and the value of microvessel density （MVD） were studied in 22 prostatic carcinoma patients using immunohistochemistry. Results： Positive expression of TSP-1 protein detected inl6 of the 22 （72.7%） cases. Most positive staining for TSP-1 was seen in the cytoplasm of the cancer cells, some was in the extracellular matrix. The mean MVD in 22 cases with prostatic carcinoma was 71.21±31.14 vessels per 100 field of vision. Tumors with a high expression of TSP-1 showed a high value of MVD and the correlation between TSP-1 immunocompetence and microvessel density was highly significant （r=0.54, P=0.009）. Conclusions： The TSP-1 is strongly expressed in most prostatic carcinoma and were associated with neovascularization-1 expression might TSP-1 is likely to contribute to the extensive neovascularization and increased TSP- participate in angiogenic phenotype in prostatic carcinoma.