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NIRF对P53蛋白泛素化作用的研究 被引量：12

Studies on The Interactions Between NIRF and P53
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摘要 HEK293和HeLa细胞分别被Np95/ICBP90-likeRINGfingerprotein(NIRF)和P53转染后,细胞上清和免疫沉淀产物用SDS-聚丙烯酰胺凝胶电泳免疫印迹法分析;细菌合成GST-P53后,用GSTpull-down技术检测NIRF与P53相互作用;在GST-P53、E1、E2和NIRF体外泛素化反应系统中,检测NIRF对P53的体外泛素化.结果表明:NIRF能与P53相互作用,NIRF不仅能与P53特异性结合,而且还会将P53泛素化,这种相互作用在细胞内和细胞外均能发生.推测NIRF可能是P53的一个新的负调节蛋白. HEK293 or HeLa cells were transfected by N1RF and, or P53, whole cell extracts and immunoprecipitates were subjected to SDS-PAGE followed by Western blotting. GST pull-down was carried out to identify the interactions between NIRF and P53. In vitro ubiquitination reaction was carried out to identify P53 ubquitinate by NIRF. The results suggested that NIRF could interact with P53 in vivo and in vitro. The results also showed that NIRF could ubiquitinate P53 in vivo and in vitro. The results indicated that NIRF would be a new negative regulator of P53.
作者 段昌柱 蒲淑萍 TSUTOMU MORI HIDEO KOCHI 邱宗荫
机构地区 重庆医科大学细胞生物学与遗传学教研室 The 重庆医科大学医学检验系
出处 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2006年第2期163-168,共6页 Progress In Biochemistry and Biophysics
基金 日元贷款培训项目资助.~~
关键词 NIRF P53 GST pull-down 泛素化 NIRF, P53, GST pull-down, ubiquitination
分类号 R735 [医药卫生—肿瘤] Q26 [生物学—细胞生物学]
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参考文献13

  • 1Li Y,Mori T,Hata H,et al.NIRF induces G1 arrest and associates with Cdk2.Biochem Biophys Res Commun,2004,319 (2):464 ～468.
  • 2Mori T,Li Y,Hata H,et al.NIRF,a novel RING finger protein,is involved in cell-cycle regulation.Biochem Biophys Res Commun,2002,296 (3):530～536.
  • 3Mori T,Li Y,Hata H,et al.NIRF is a ubiquitin ligase that is capable of ubiquitinating PCNP,a PEST-containing nuclear protein.FEBS Lett,2004,557 (1 ～3):209～214.
  • 4Moll U M,Petrenko O.The MDM2-p53 interaction.Mol Cancer Res,2003,1 (14):1001 ～1008.
  • 5Shmueli A,Oren M.Regulation of p53 by Mdm2:fate is in the numbers.Mol Cell,2004,13 (1):4～5.
  • 6Vogelstein B,Lane D,Levine A J.Surfing the p53 network.Nature,2000,408 (6810):307 ～ 310.
  • 7Yang Y,Li C C,Weissman A M.Regulating the p53 system through ubiquitination.Oncogene,2004,23 (11):2096～2106.
  • 8Pickart C M,Eddins M J.Ubiquitin:structures,functions,mechanisms.Biochim Biophys Acta,2004,1695 (1 ～3):55～72.
  • 9Smalle J,Vierstra R D.The ubiquitin 26 S proteasome proteolytic pathway.Annu Rev Plant Biol,2004,55:555 ～590.
  • 10Roos-Mattjus P,Sistonen L.The ubiquitin-proteasome pathway.Ann Med,2004,36 (4):285～295.

同被引文献82

  • 1许良中,杨文涛.免疫组织化学反应结果的判断标准[J].中国癌症杂志,1996,6(4):229-231. 被引量：1367
  • 2应若素,范学工,李宁,朱才,刘洪波.流体动力学法乙型肝炎病毒感染动物模型的建立[J].世界华人消化杂志,2006,14(11):1052-1057. 被引量：5
  • 3Anneke Funk Mouna Mhamdi Hans Will Hüseyin Sirma.Avian hepatitis B viruses： Molecular and cellular biology, phylogenesis, and host tropism[J].World Journal of Gastroenterology,2007,13(1):91-103. 被引量：16
  • 4Mori T, Li Y, Hata H, et al. NIRF is a ubiquitin ligase that is capable of ubiquitinating PCNP, a PEST-containing nuclear protein. FEBS Lett, 2004, 557( 1-3 ): 209-214.
  • 5Brommer C, Achour M, Arima Y, et al. The UHRF family: oncogenes that are drugable targets for cancer therapy in the near future. Pharmacol Ther, 2007, 115(3): 419-434.
  • 6Firth AE, Atkins JF. Bioinformatie analysis suggests that the Cypovirus 1 major core protein cistron harbours an overlapping gene. Virol J, 2008, 5( 1 ): 62.
  • 7Firth AE.Bioinformatic analysis suggests that the Orbivirus VP6 cistron encodes an overlapping gene. Virol J, 2008, 5( 1 ): 48.
  • 8Cui Y, Peng J, Zhou P, et al. Bioinformatic studies on the group 2 allergens of Dermatophagoides farinae from China. Asian Pac J Allergy Immunol, 2007, 25(4): 199-206.
  • 9Li Y, Mori T, Hata H, et al. NIRF induces G1 arrest and associates with Cdk2. Biochem Biophys Res Commun, 2004, 319 (2): 464- 468.
  • 10Mori T, Li Y, Hata H,et al. NIRF, a novel RING finger protein, is involved in cell-cycle regulation. Bioehem Biophys Res Commun, 2002, 296(3): 530-536.

引证文献12

二级引证文献19

生物化学与生物物理进展
2006年 第2期

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