健康老年人口腔念珠菌与义齿修复的相关研究

Correlation of Oral Candida spp. and Denture- wearing in Healthy Elderly

目的研究健康老年人群口腔念珠菌的定植状况,分析义齿修复对老年人口腔念珠菌定植的影响。方法212例健康老年人分为4组:A1组(男性,有义齿),B1组(男性,无义齿),A2组(女性,有义齿)和B2组(女性,无义齿)。标准含潄法取样,样本接种于沙堡琼脂培养基培养念珠菌,CHROMagarCandidaTM鉴定培养基鉴定白色念珠菌,碳水化合物同化反应鉴定体系鉴定念珠菌菌种。培养基中念珠菌菌落计数为每个样本的念珠菌检出强度。比较4组健康老年人念珠菌检出率和检出强度有无统计学差异。结果212例老年人中116例检出念珠菌,检出率为54.72%。检出念珠菌包括白色念珠菌、近平滑念珠菌、克柔念珠菌、热带念珠菌等10个菌种。A1、B1、A2和B2组念珠菌检出率分别为66.67%,36.07%,64.15%和56.00%;白色念珠菌检出率分别为56.25%,21.31%,56.60%和38.00%。A1组念珠菌、白色念珠菌检出率高于B1组(P<0.05)。高念珠菌强度者在A1、A2组所占比例分别较B1、B2组高(P<0.05)。结论健康老年人口腔总念珠菌及白色念珠菌检出率和检出强度增高与义齿修复相关。老年人口腔念珠菌检出率和检出强度的差异主要是白色念珠菌检出率和检出强度的差异所造成。

Objective To investigate the frequency and of the healthy elderly in Chengdu, and to study the role intensity of Candida spp. incidence from the oral cavities of the dentures in the distribution of oral Candida spp. Methods A total of 212 individuals （age 〉60 years） were divided into four groups： A1 （48 man with dentures）, B1 （61 man without dentures）, A2（53 women with dentures） and B2（50 women without dentures）. Samples of their oral flora were obtained by rinsing with 10 mL PBS solution. The samples were centrifuged and resuspended in PBS （500 IxL）, and plated onto Sabouraud＇s dextrose agar. CHROMagar CandidaTM, sugar assimilation patterns （API 20C AUX tests） were used to determine Candida spp. The total number of yeast colonies on the plates was considered as the relative intensity of oral Candida. Results Candida spp. was isolated from 116 healthy elderly individuals （54.72%）, such as C.albicans, C.parapsilosis, C. krusei, C.guilliermondii, C.tropicalis, etc. The frequency of Cand/da spp. in A1, B1, A2 and B2 was 66.67%, 36.07%, 64.15%, and 56.00%, respectively. The frequency of C. albicans in A1, B1, A2 and B2 was 56.25%, 21.31%, 56.60% and 38.00%, respectively. The frequency of Candida spp. and the intensity of Candida spp. were greater for individuals in the denture-wearing group than that in the control. Conclusion The frequency and intensity of Candida spp. incidence from the healthy elderly are closely correlated with denture-wearing, and the differences of the frequency and intensity of Candida spp. incidence in the elderly are due to the differences of frequency and intensity of C.albicans incidence.